中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2013年
3期
299-301
,共3页
哌啶类%受体,N-甲基-D-天冬氨酸%药物耐受性
哌啶類%受體,N-甲基-D-天鼕氨痠%藥物耐受性
고정류%수체,N-갑기-D-천동안산%약물내수성
Piperidines%Receptors,N-methyl-D-aspartate%Drug tolerance
目的 探讨瑞芬太尼和芬太尼对大鼠脊髓背角神经元NMDA受体通道电流的影响.方法 采用全细胞膜片钳技术记录NMDA受体通道电流.原代培养的E14SD大鼠脊髓背角神经元(DH细胞)30个,采用随机数字表法,将其分为3组(n=10):瑞芬太尼组(R组)、芬太尼组(F组)、对照组(C组).4 nmol/L瑞芬太尼(R组)、10 μmol/L芬太尼(F组)灌流DH细胞60 min后洗脱.于给药后即刻(T0)、药物作用15 min(T1)、30 min(T2)、45 min(T3)、60 min(T4)、洗脱后15 min(T5)、30 min(T6)时记录NMDA受体通道电流.结果 与C组比较,F组各时点NMDA受体通道峰电流差异无统计学意义,R组T0、T1时NMDA受体通道峰电流差异无统计学意义(P＞0.05),T2-T6时NMDA受体通道峰电流升高(P＜0.01)；与T0时比较,R组T3-T6时NMDA受体通道峰电流升高(P＜0.01)；与T5时比较,R组T2-T4时、T6时NMDA峰电流下降(P＜0.01).结论 瑞芬太尼可增强大鼠脊髓背角神经元NMDA受体功能,于洗脱后达峰效应,芬太尼无此作用.
目的 探討瑞芬太尼和芬太尼對大鼠脊髓揹角神經元NMDA受體通道電流的影響.方法 採用全細胞膜片鉗技術記錄NMDA受體通道電流.原代培養的E14SD大鼠脊髓揹角神經元(DH細胞)30箇,採用隨機數字錶法,將其分為3組(n=10):瑞芬太尼組(R組)、芬太尼組(F組)、對照組(C組).4 nmol/L瑞芬太尼(R組)、10 μmol/L芬太尼(F組)灌流DH細胞60 min後洗脫.于給藥後即刻(T0)、藥物作用15 min(T1)、30 min(T2)、45 min(T3)、60 min(T4)、洗脫後15 min(T5)、30 min(T6)時記錄NMDA受體通道電流.結果 與C組比較,F組各時點NMDA受體通道峰電流差異無統計學意義,R組T0、T1時NMDA受體通道峰電流差異無統計學意義(P＞0.05),T2-T6時NMDA受體通道峰電流升高(P＜0.01)；與T0時比較,R組T3-T6時NMDA受體通道峰電流升高(P＜0.01)；與T5時比較,R組T2-T4時、T6時NMDA峰電流下降(P＜0.01).結論 瑞芬太尼可增彊大鼠脊髓揹角神經元NMDA受體功能,于洗脫後達峰效應,芬太尼無此作用.
목적 탐토서분태니화분태니대대서척수배각신경원NMDA수체통도전류적영향.방법 채용전세포막편겸기술기록NMDA수체통도전류.원대배양적E14SD대서척수배각신경원(DH세포)30개,채용수궤수자표법,장기분위3조(n=10):서분태니조(R조)、분태니조(F조)、대조조(C조).4 nmol/L서분태니(R조)、10 μmol/L분태니(F조)관류DH세포60 min후세탈.우급약후즉각(T0)、약물작용15 min(T1)、30 min(T2)、45 min(T3)、60 min(T4)、세탈후15 min(T5)、30 min(T6)시기록NMDA수체통도전류.결과 여C조비교,F조각시점NMDA수체통도봉전류차이무통계학의의,R조T0、T1시NMDA수체통도봉전류차이무통계학의의(P＞0.05),T2-T6시NMDA수체통도봉전류승고(P＜0.01)；여T0시비교,R조T3-T6시NMDA수체통도봉전류승고(P＜0.01)；여T5시비교,R조T2-T4시、T6시NMDA봉전류하강(P＜0.01).결론 서분태니가증강대서척수배각신경원NMDA수체공능,우세탈후체봉효응,분태니무차작용.
Objective To investigate the effect of remifentanil and fentanyl on N-methyl-D-aspartate (NMDA) receptor currents in rat spinal cord dorsal horn neurons.Methods Whole-cell patch-clamp technique was used to record the NMDA receptor currents.The primary cultured E14SD rat spinal cord dorsal horn neurons (DH cells) were randomly divided into 3 groups (n =10 each):remifentanil group (group R),fentanyl group (group F) and control group (group C).DH cells were perfused with 4 nmol/L remifentanil (group R) or 10 μmol/L fentanyl (group F) for 60 min followed by washout.NMDA receptor currents were recorded immediately after administration (T0),at 15,30,45 and 60 min of action of drugs (T1-4),and at 15 and 30 min (T5-6) after washout.Results Compared with group C,no significant change in the peak NMDA receptor current was found at each time point in group F and at T0 and T1 in group R (P ＞ 0.05),and the peak NMDA receptor current was significantly increased at T2-6 in group R (P ＜ 0.01).The peak NMDA receptor current was significantly higher at T2-6 than at T0,while lower at T2-4 and T6 than at T5 in group R (P ＜ 0.01).Conclusion Remifentanil can increase NMDA receptor function in rat spinal cord horn neurons,and the peak effect is reached after washout,but fentanyl dose not have the effect.
