CAJ | 학술논문

采用单独使用二乙烯亚胺（BEI）灭活剂30℃、26℃灭活以及二乙烯亚胺-甲醛（BEI-FA）联合灭活3种方法对悬浮培养生产的猪口蹄疫O型ZK/93病毒液进行灭活,考察和评价各种方法对该毒株146S的影响及灭活安全性。根据对3批口蹄疫病毒灭活后检测结果来看,单独使用BEI 26℃灭活方法虽然对146S的损失率小,但存在一定的安全隐患,未经过大量试验证实及确认之前不应使用。而经BEI-FA联合灭活方法获得的抗原安全性检验符合规定,但该方法对口蹄疫抗原146S的损失率较高,不宜推广和使用。与上述两种方法相比,用BEI灭活剂30℃灭活28 h仍是最佳的灭活方法。
채용단독사용이을희아알（BEI）멸활제30℃、26℃멸활이급이을희아알-갑철（BEI-FA）연합멸활3충방법대현부배양생산적저구제역O형ZK/93병독액진행멸활,고찰화평개각충방법대해독주146S적영향급멸활안전성。근거대3비구제역병독멸활후검측결과래간,단독사용BEI 26℃멸활방법수연대146S적손실솔소,단존재일정적안전은환,미경과대량시험증실급학인지전불응사용。이경BEI-FA연합멸활방법획득적항원안전성검험부합규정,단해방법대구제역항원146S적손실솔교고,불의추엄화사용。여상술량충방법상비,용BEI멸활제30℃멸활28 h잉시최가적멸활방법。
In order to investigate and evaluate the effect on 146S and the security of inactivation,three different inactivation methods were respectively used to inactivate the O type ZK/93 strain of foot and mouth disease virus produced by suspension culture.These three inactivation methods were BEI inactivation alone at 30 ℃,BEI inactivation alone at 26 ℃ and BEI-FA combined inactivation.From the test result of three batches of inactivation,we can conclude that the loss rate of 146S was small using BEI alone at 26 ℃,but the security risk existed.This method should not be used before it is proved and confirmed by a lot of tests.The method of combined BEI-FA had high security,but the loss rate of 146S was high and should not be promoted and used.Comparing with two methods above,BEI alone at 30 ℃ for 28 hours is still the best method.