CAJ | 학술논문

目的:探讨慢性乙型肝炎(CHB)患者外周血乙型肝炎病毒(HBV)特异性细胞毒性T淋巴细胞(CTLs)表面CD244与程序性死亡蛋白1(PD-1) 的共表达模式及其与疾病进展的关系.方法:采集81例CHB患者和14例健康者外周血,分离外周血单个核细胞(PBMC);利用主要组织相容性复合体I(MHC-I)-肽五聚体技术标定HBV表位特异性CTLs;荧光抗体标记细胞表面CD244与PD-1分子,流式细胞术检测.结果:CHB患者CD244与PD-1在HBV特异性CTLs表面共表达较总CD8+T细胞表面明显升高(67.48% ± 17.16% vs 14.01% ± 7.97%,P<0.01);HBV感染的不同临床类型中,轻中度的CHB患者HBV特异性CTLs表面CD244与PD-1共表达水平较免疫耐受者明显升高(72.05% ± 16.86% vs 53.11% ± 18.05%,P<0.05),肝衰竭组CD244与PD-1共表达水平较轻中度CHB下调(63.11% ± 13.87% vs 72.05% ± 16.86%,P<0.05)并伴随干扰素γ(IFN-γ)分泌功能的恢复(30.95% ± 20.29% vs 13.63% ± 10.46%,P<0.01).结论:CHB患者 HBV特异性CTLs细胞表面高共表达CD244与PD-1分子,CD244与PD-1共表达在CHB不同临床类型中有差异,并与IFN-γ分泌水平呈负相关,提示CD244可能与CHB患者HBV特异性CTLs功能低下及CHB疾病进展相关.
목적:탐토만성을형간염(CHB)환자외주혈을형간염병독(HBV)특이성세포독성T림파세포(CTLs)표면CD244여정서성사망단백1(PD-1) 적공표체모식급기여질병진전적관계.방법:채집81례CHB환자화14례건강자외주혈,분리외주혈단개핵세포(PBMC);이용주요조직상용성복합체I(MHC-I)-태오취체기술표정HBV표위특이성CTLs;형광항체표기세포표면CD244여PD-1분자,류식세포술검측.결과:CHB환자CD244여PD-1재HBV특이성CTLs표면공표체교총CD8+T세포표면명현승고(67.48% ± 17.16% vs 14.01% ± 7.97%,P<0.01);HBV감염적불동림상류형중,경중도적CHB환자HBV특이성CTLs표면CD244여PD-1공표체수평교면역내수자명현승고(72.05% ± 16.86% vs 53.11% ± 18.05%,P<0.05),간쇠갈조CD244여PD-1공표체수평교경중도CHB하조(63.11% ± 13.87% vs 72.05% ± 16.86%,P<0.05)병반수간우소γ(IFN-γ)분비공능적회복(30.95% ± 20.29% vs 13.63% ± 10.46%,P<0.01).결론:CHB환자 HBV특이성CTLs세포표면고공표체CD244여PD-1분자,CD244여PD-1공표체재CHB불동림상류형중유차이,병여IFN-γ분비수평정부상관,제시CD244가능여CHB환자HBV특이성CTLs공능저하급CHB질병진전상관.