CAJ | 학술논문

采用Design-Expert软件进行响应面试验(RSM)设计，利用外循环蒸煮锅对桉木片半纤维素自催化水解制取还原糖方法进行了初步探索。结果表明反应温度、保温时间交互作用对还原糖制取量影响显著，当水解因子P<2500时，响应面模型/水解P因子模型对于还原糖提取率R的影响一致，即R (153℃,90 min)≈R(162℃,30 min)，R (158℃,90 min)≈R (168℃,30 min)，R (164℃,90 min)≈R (176℃,30 min)。P因子在1000~1500范围内水解液中低聚木糖及木糖含量最高，总浓度为9.7 g·L-1，在反应温度170℃、保温40 min(P因子约1000)条件下达到最优水平。当P因子>2000时糖组分总量呈现下降趋势，通过FT-IR、GC-MS分析，单糖组分大量转化为糠醛等其他降解产物。
채용Design-Expert연건진행향응면시험(RSM)설계，이용외순배증자과대안목편반섬유소자최화수해제취환원당방법진행료초보탐색。결과표명반응온도、보온시간교호작용대환원당제취량영향현저，당수해인자P<2500시，향응면모형/수해P인자모형대우환원당제취솔R적영향일치，즉R (153℃,90 min)≈R(162℃,30 min)，R (158℃,90 min)≈R (168℃,30 min)，R (164℃,90 min)≈R (176℃,30 min)。P인자재1000~1500범위내수해액중저취목당급목당함량최고，총농도위9.7 g·L-1，재반응온도170℃、보온40 min(P인자약1000)조건하체도최우수평。당P인자>2000시당조분총량정현하강추세，통과FT-IR、GC-MS분석，단당조분대량전화위강철등기타강해산물。
This response surface methodology experiment was designed to include the three easy-to-control independent variables the liquid-solid ratio, the highest temperature and the holding time, based on the Box-Behnken experimental design principles using Design-Expert software. When hydrolysis factor (P) was kept below 2 500, the effect under low temperatureswith long holding timeswereapproximately equal to that under high temperatureswith short holding times for reducing sugar extraction rate (R): R (153oC, 90 min) ≈R (162oC, 30 min), R (158oC, 90 min)≈R (168oC, 30 min), R (164oC, 90 min)≈R (176oC, 30 min). With P-factor in this range, a linear relationship was found between this factor P and the response value R (reducing sugar/oven-dry wood, g·g-1). It was noteworthy that there was more xylo-oligosaccharide than xylose with the P-factor ranging from about 1000 to 1500, and it was also observed that an extraction temperature of 170oC provided better conversion of valuable xylo-oligosaccharide (about 55%) and xylose in wood-based bio-refinery system, especially under the condition of auto-hydrolysis for 40 minutes and P-factor about 1000. Combined utilization of fourier transformed infrared spectrometry (FT-IR) and gas chromatography-mass spectrometry (GC-MS) proved useful for establishing detailed mass balances of the auto-hydrolysis process. Analyses of results obtained showed that with the increase of auto-hydrolysis intensity, low degree polymerizedxylan can be further converted into small quantities of furfural and other degradation products.