CAJ | 학술논문

目的：探讨外周血癌胚抗原信使核糖核酸（CEAmRNA）、中性粒细胞多肽1-3（HNP1-3）单一检测及联合检测在结直肠癌诊断中价值。方法：选取结直肠癌70例患者为观察组，另选良性疾病患者70例为对照组。分别采用Nested RT-PCR法检测CEAmRNA，ELISA法检测HNP1-3，观察两组患者CEAmRNA及HNP1-3的表达。结果：CEAmRNA对结直肠癌的诊断敏感度为52.86%，特异度为98.57%；HNP1-3对结直肠癌的诊断敏感度为60%，特异度为98.57%。两者联合检测时至少1项阳性敏感度为77.14%，特异度为97.14%，2项均阳性敏感度为42.85%，特异度为100%。CEAmRNA、HNP1-3的表达与淋巴结转移及Dukes分期相关。结论：在结直肠癌诊断中，外周血CEAmRNA和HNP1-3二者平行相关检测具有更高价值和诊断意义。
목적：탐토외주혈암배항원신사핵당핵산（CEAmRNA）、중성립세포다태1-3（HNP1-3）단일검측급연합검측재결직장암진단중개치。방법：선취결직장암70례환자위관찰조，령선량성질병환자70례위대조조。분별채용Nested RT-PCR법검측CEAmRNA，ELISA법검측HNP1-3，관찰량조환자CEAmRNA급HNP1-3적표체。결과：CEAmRNA대결직장암적진단민감도위52.86%，특이도위98.57%；HNP1-3대결직장암적진단민감도위60%，특이도위98.57%。량자연합검측시지소1항양성민감도위77.14%，특이도위97.14%，2항균양성민감도위42.85%，특이도위100%。CEAmRNA、HNP1-3적표체여림파결전이급Dukes분기상관。결론：재결직장암진단중，외주혈CEAmRNA화HNP1-3이자평행상관검측구유경고개치화진단의의。
Objective: To explore the diagnosis of colorectal cancer by the single detection,parallel and series detection with peripheral blood CEAmRNA and HNP1-3. Methods: The expression of CEAmRNA was detected by using Nested RT-PCR in 70 patients with colorectal cancer (the experimental group) and in 70 normal subjects (the control group). The expression of HNP1-3 was detected by using ELISA in both groups. Results:The sensitivity of CEAmRNA and HNP1-3 in the experimental group was 52.86% and 60%, respectively. The specificity of CEAmRNA and HNP1-3 in the experimental group was 98.57%. A higher sensitivity (77.14%)was shown when either of the CEAmRNA and HNP1-3 was positive. A higher specificity(100%)was found when both of the CEAmRNA and HNP1-3 were positive. The expressions of CEAmRNA and HNP1-3 were not significantly correlated with the patient’s sex, age, position of tumor or differentiation level of cancer cells, but with the clinical stage and lymph node metastasis. Conclusion:In the diagnosis of colorectal cancer, the single detection plays a certain role. But the parallel and series detections are more valuable and diagnostically significant.