CAJ | 학술논문

目的：通过观察特定浓度的转化生长因子β1(TGF-β1)，在不同时间刺激体外培养种植体表面成骨细胞中I型胶原蛋白表达情况，探讨TGF-β1对种植体表面成骨细胞增殖分化的影响。方法：体外培养成骨细胞模拟种植体植入后的体内环境接种于钛片表面,将其分为2个浓度刺激组和对照组。刺激组分别加入0.5ng/mL、10ng/mL的TGF-β1刺激细胞增殖和分化，分别收集刺激后第2d、5d、7d培养的细胞。通过RT-PCR检测不同培养时间成骨细胞中的I型胶原蛋白mRNA表达，对照组不加TGF-β1，比较各组间差异程度。结果：浓度为0.5ng/mL的外源性TGF-β1加入到接种于模拟体内种植体的钛片表面的成骨细胞中后，可显著增加成骨细胞中I型胶原蛋白表达(P<0.05)；而浓度为10ng/mL的外源性TGF-β1可降低成骨细胞中I型胶原蛋白的表达(P<0.05)。TGF-β1刺激后的I型胶原蛋白在不同时间培养的成骨细胞中，表达在凝胶成像系统光密度分析后可见与对照组中表达量相比，低浓度刺激组表达量略有升高，高浓度刺激组表达量略有降低。结论：浓度为0.5ng/mL的TGF-β1对I型胶原蛋白的表达有促进作用；浓度为10ng/mL外源性TGF-β1对I型胶原蛋白表达有抑制作用。
목적：통과관찰특정농도적전화생장인자β1(TGF-β1)，재불동시간자격체외배양충식체표면성골세포중I형효원단백표체정황，탐토TGF-β1대충식체표면성골세포증식분화적영향。방법：체외배양성골세포모의충식체식입후적체내배경접충우태편표면,장기분위2개농도자격조화대조조。자격조분별가입0.5ng/mL、10ng/mL적TGF-β1자격세포증식화분화，분별수집자격후제2d、5d、7d배양적세포。통과RT-PCR검측불동배양시간성골세포중적I형효원단백mRNA표체，대조조불가TGF-β1，비교각조간차이정도。결과：농도위0.5ng/mL적외원성TGF-β1가입도접충우모의체내충식체적태편표면적성골세포중후，가현저증가성골세포중I형효원단백표체(P<0.05)；이농도위10ng/mL적외원성TGF-β1가강저성골세포중I형효원단백적표체(P<0.05)。TGF-β1자격후적I형효원단백재불동시간배양적성골세포중，표체재응효성상계통광밀도분석후가견여대조조중표체량상비，저농도자격조표체량략유승고，고농도자격조표체량략유강저。결론：농도위0.5ng/mL적TGF-β1대I형효원단백적표체유촉진작용；농도위10ng/mL외원성TGF-β1대I형효원단백표체유억제작용。
Objective:To investigate the cosnsequential changes of collagen-I gene expressions of osteoblasts on the implant surfaces incubated with transforming growth factor-beta1, and to make a primary understanding of functions of proliferation and differentiation. Methods: The osteoblasts were seeded on the surfaces of the implant (the Ti disc was made into the model implant). The cells were divided into two induced groups and the control group. To stimulate the impact of the collection after 2d, 5d and 7d, cells were cultured respectively. The expressions of mRNA were detected by reverse transcription-polymerase chain reaction (RT-PCR). Experiments were divided into the two levels of stimulated groups and one control group. The cells were collected after culture of 2d, 5d and 7d . By using RT-PCR, the cells showed collagen-I gene expression and comparative analysis was conducted. All the data were analyzed by SPSS16.0 software. After the comparison of the degrees of differences between the groups, statistically significant difference was shown (P<0.05). Results: The extraneous transforming growth factor-beta 1 (0.5ng/ml) increased the collagen-I (COL-I) gene expressions of osteoblasts on the surfaces of the implant (the Ti disc was made into the model implant)(P<0.05), while the extraneous transforming growth factor-beta1 (10ng/ml) decreased the collagen-I(COL-I) gene expressions of osteoblasts (P<0.05). The extraneous transforming growth factor-beta1 stimulation of COL-I at different periods of time in the osteoblasts in the gel imaging system, optical density analysis showed that the expression of the control group compared to the low concentration stimulated group increased slightly and the high concentration stimulated group decreased slightly.Conclusion:It is concluded that 0.5ng/ml extraneous transforming growth factor-beta 1 increased collagen-I (COL-I) gene expressions in the osteoblasts on the surfaces of the implant (the Ti disc was made into the model implant) , while 10ng/ml extraneous transforming growth factor-beta 1 decreased gene COL-I expressions.