交通医学
交通醫學
교통의학
MEDICAL JOURNAL OF COMMUNICATIONS
2013年
5期
435-438
,共4页
张晓义%唐祝奇%刘春%朱晓晖%印彤%王粹芳%崔世维
張曉義%唐祝奇%劉春%硃曉暉%印彤%王粹芳%崔世維
장효의%당축기%류춘%주효휘%인동%왕수방%최세유
糖尿病足%血管内皮生长因子%皮肤溃疡%蟾皮%大鼠%动物模型%酶联免疫吸附试验
糖尿病足%血管內皮生長因子%皮膚潰瘍%蟾皮%大鼠%動物模型%酶聯免疫吸附試驗
당뇨병족%혈관내피생장인자%피부궤양%섬피%대서%동물모형%매련면역흡부시험
Diabetic Foot%vascular endothelial growth factor%Dermal Ulcer%Toad skin%rats%animal model%en-zyme-linked immunosorbent assay
目的：研究鲜蟾皮外敷对糖尿病大鼠皮肤溃疡的治疗作用及作用机制。方法：清洁级雄性SD大鼠42只，分为3组：正常对照组（12只）、糖尿病皮肤溃疡模型组（15只）、糖尿病皮肤溃疡治疗组（15只）。建立缺损性皮肤溃疡大鼠模型，鲜蟾皮外敷溃疡部位，观察溃疡愈合情况；大体病理观察第2、3周末溃疡组织结构，免疫组化观察血管内皮生长因子（VEGF）的表达，酶联免疫反应技术测定组织中VEGF含量。结果：模型组2周溃疡开始缩小，3周结痂，与对照组相比VEGF表达降低（15.6±2.2μg/g vs.48.3±4.2μg/g, P<0.05）；与模型组相比，治疗组2周VEGF表达增加（40.7±4.5μg/g vs.15.6±2.2μg/g, P<0.05），持续到3周（19.5±3.2μg/g vs.10.9±2.7μg/g, P<0.05），创面愈合周期提前。结论：鲜蟾皮外敷可增加VEGF表达，促进糖尿病大鼠皮肤溃疡愈合。
目的：研究鮮蟾皮外敷對糖尿病大鼠皮膚潰瘍的治療作用及作用機製。方法：清潔級雄性SD大鼠42隻，分為3組：正常對照組（12隻）、糖尿病皮膚潰瘍模型組（15隻）、糖尿病皮膚潰瘍治療組（15隻）。建立缺損性皮膚潰瘍大鼠模型，鮮蟾皮外敷潰瘍部位，觀察潰瘍愈閤情況；大體病理觀察第2、3週末潰瘍組織結構，免疫組化觀察血管內皮生長因子（VEGF）的錶達，酶聯免疫反應技術測定組織中VEGF含量。結果：模型組2週潰瘍開始縮小，3週結痂，與對照組相比VEGF錶達降低（15.6±2.2μg/g vs.48.3±4.2μg/g, P<0.05）；與模型組相比，治療組2週VEGF錶達增加（40.7±4.5μg/g vs.15.6±2.2μg/g, P<0.05），持續到3週（19.5±3.2μg/g vs.10.9±2.7μg/g, P<0.05），創麵愈閤週期提前。結論：鮮蟾皮外敷可增加VEGF錶達，促進糖尿病大鼠皮膚潰瘍愈閤。
목적：연구선섬피외부대당뇨병대서피부궤양적치료작용급작용궤제。방법：청길급웅성SD대서42지，분위3조：정상대조조（12지）、당뇨병피부궤양모형조（15지）、당뇨병피부궤양치료조（15지）。건립결손성피부궤양대서모형，선섬피외부궤양부위，관찰궤양유합정황；대체병리관찰제2、3주말궤양조직결구，면역조화관찰혈관내피생장인자（VEGF）적표체，매련면역반응기술측정조직중VEGF함량。결과：모형조2주궤양개시축소，3주결가，여대조조상비VEGF표체강저（15.6±2.2μg/g vs.48.3±4.2μg/g, P<0.05）；여모형조상비，치료조2주VEGF표체증가（40.7±4.5μg/g vs.15.6±2.2μg/g, P<0.05），지속도3주（19.5±3.2μg/g vs.10.9±2.7μg/g, P<0.05），창면유합주기제전。결론：선섬피외부가증가VEGF표체，촉진당뇨병대서피부궤양유합。
Objective: To study the effect of the treatment of skin ulcers in diabetic rats by external application of fresh toad skin and its possible mechanism of action. Methods: 42 male SD rats were randomly divided into three groups:the normal control group (12 rats), the diabetic skin ulcers model group (15 rats), and the diabetic skin ulcers treatment group (15 rats). A model of skin ulcers in rats was created, then fresh toad skin was applied on these ulcer sites, and how the ulcers got healed was observed. Pathologically, the wounded tissue structures around the ulcers were observed at the 2nd and 3rd weekends, and immunohistochemically, the expression of the wounded tissue vascular endothelial growth factor (VEGF) was observed. Results: Ulcers began to shrink after 2 weeks and scab after 3 weeks in the diabetic model group. By contrast, in the diabetic treatment group, the expression of VEGF was increased (15.6±2.2 μg/g vs. 48.3±4.2 μg/g, P<0.05) at the end of 2 weeks and sustained to the end of 3 weeks (19.5±3.2μg/g vs. 10.9±2.7μg/g, P<0.05), and meanwhile the wound healing cycle was shortened. Conclusions:External application of fresh toad skin can increase the expression of the wounded tissues VEGF and promote the healing of skin ulcers in diabetic rats.
