交通医学
交通醫學
교통의학
MEDICAL JOURNAL OF COMMUNICATIONS
2013年
5期
425-428,432
,共5页
冯金荣%孙伟%庄重%朱丹丹%段义农
馮金榮%孫偉%莊重%硃丹丹%段義農
풍금영%손위%장중%주단단%단의농
白念珠菌%菌丝发育%蛋白磷酸酯酶CaPph3%调节亚基CaPsy2%羟基脲%甲基磺酸甲酯
白唸珠菌%菌絲髮育%蛋白燐痠酯酶CaPph3%調節亞基CaPsy2%羥基脲%甲基磺痠甲酯
백념주균%균사발육%단백린산지매CaPph3%조절아기CaPsy2%간기뇨%갑기광산갑지
Candida albicans%Filamentous growth%protein phosphatase CaPph3%regulatory subunit CaPsy2%HU%MMS
目的：研究白念珠菌蛋白磷酸酯酶CaPph3及调节亚基CaPsy2在调控白念珠菌菌丝发育过程中的作用。方法：利用遗传毒性试剂HU和MMS分别处理CaPPH3和CaPSY2缺失株，观察移除毒性试剂后缺失株的菌丝发育情况。结果：（1）缺失株的表型鉴定：在含有遗传毒性试剂MMS、HU以及CP平板上，与野生型菌株RM1000相比， CaPPH3缺失株、CaPSY2缺失株和CaPPH3 CaPSY2双缺失株均表现出明显生长缺陷，且各缺失株间差异不大。（2）缺失株对遗传毒性试剂敏感度测定：用MMS和HU处理各菌株后，野生型菌株的存活率约95%，而CaPPH3缺失株、CaPSY2缺失株和CaPPH3 CaPSY2双基因缺失株存活率明显下降，且相互之间差异不大。（3）缺失株菌丝发育实验：用0.020%MMS处理各菌株4小时后，野生型菌株RM1000还维持酵母态，而CaPPH3缺失株、CaPSY2缺失株和CaPPH3 CaPSY2双基因缺失株已出芽形成菌丝；而移除MMS后野生型菌株形成菌丝，6小时后不再延长，在菌丝顶端形成酵母态细胞，而CaPPH3、CaPSY2和CaPPH3 CaPSY2双基因缺失株仍维持菌丝态。用40 mmol/L HU处理各菌株4小时后，各菌株都维持酵母态；而移除HU后野生型菌株仍维持酵母态，而CaPPH3缺失株、CaPSY2缺失株和CaPPH3 CaPSY2双基因缺失株则会逐渐形成菌丝。结论：CaPph3和CaPsy2参与调节遗传毒性试剂诱导的白念珠菌的菌丝发育。
目的：研究白唸珠菌蛋白燐痠酯酶CaPph3及調節亞基CaPsy2在調控白唸珠菌菌絲髮育過程中的作用。方法：利用遺傳毒性試劑HU和MMS分彆處理CaPPH3和CaPSY2缺失株，觀察移除毒性試劑後缺失株的菌絲髮育情況。結果：（1）缺失株的錶型鑒定：在含有遺傳毒性試劑MMS、HU以及CP平闆上，與野生型菌株RM1000相比， CaPPH3缺失株、CaPSY2缺失株和CaPPH3 CaPSY2雙缺失株均錶現齣明顯生長缺陷，且各缺失株間差異不大。（2）缺失株對遺傳毒性試劑敏感度測定：用MMS和HU處理各菌株後，野生型菌株的存活率約95%，而CaPPH3缺失株、CaPSY2缺失株和CaPPH3 CaPSY2雙基因缺失株存活率明顯下降，且相互之間差異不大。（3）缺失株菌絲髮育實驗：用0.020%MMS處理各菌株4小時後，野生型菌株RM1000還維持酵母態，而CaPPH3缺失株、CaPSY2缺失株和CaPPH3 CaPSY2雙基因缺失株已齣芽形成菌絲；而移除MMS後野生型菌株形成菌絲，6小時後不再延長，在菌絲頂耑形成酵母態細胞，而CaPPH3、CaPSY2和CaPPH3 CaPSY2雙基因缺失株仍維持菌絲態。用40 mmol/L HU處理各菌株4小時後，各菌株都維持酵母態；而移除HU後野生型菌株仍維持酵母態，而CaPPH3缺失株、CaPSY2缺失株和CaPPH3 CaPSY2雙基因缺失株則會逐漸形成菌絲。結論：CaPph3和CaPsy2參與調節遺傳毒性試劑誘導的白唸珠菌的菌絲髮育。
목적：연구백념주균단백린산지매CaPph3급조절아기CaPsy2재조공백념주균균사발육과정중적작용。방법：이용유전독성시제HU화MMS분별처리CaPPH3화CaPSY2결실주，관찰이제독성시제후결실주적균사발육정황。결과：（1）결실주적표형감정：재함유유전독성시제MMS、HU이급CP평판상，여야생형균주RM1000상비， CaPPH3결실주、CaPSY2결실주화CaPPH3 CaPSY2쌍결실주균표현출명현생장결함，차각결실주간차이불대。（2）결실주대유전독성시제민감도측정：용MMS화HU처리각균주후，야생형균주적존활솔약95%，이CaPPH3결실주、CaPSY2결실주화CaPPH3 CaPSY2쌍기인결실주존활솔명현하강，차상호지간차이불대。（3）결실주균사발육실험：용0.020%MMS처리각균주4소시후，야생형균주RM1000환유지효모태，이CaPPH3결실주、CaPSY2결실주화CaPPH3 CaPSY2쌍기인결실주이출아형성균사；이이제MMS후야생형균주형성균사，6소시후불재연장，재균사정단형성효모태세포，이CaPPH3、CaPSY2화CaPPH3 CaPSY2쌍기인결실주잉유지균사태。용40 mmol/L HU처리각균주4소시후，각균주도유지효모태；이이제HU후야생형균주잉유지효모태，이CaPPH3결실주、CaPSY2결실주화CaPPH3 CaPSY2쌍기인결실주칙회축점형성균사。결론：CaPph3화CaPsy2삼여조절유전독성시제유도적백념주균적균사발육。
Objective: To study the function of protein phosphatase CaPph3 and the regulatory subunit CaPsy2 in the regulation of the filamentous growth of Candida albicans. Methods:The cells of Candida albicans were treated with HU and MMS,and then the filamentous growth was checked by microscope after the genotoxic agents were removed. Results:(1)The phenotype of the deletion mutants:Compared with wildtype, deletion mutants of CaPPH3, CaPSY2 and the double-gene deletion mutants for CaPPH3 and CaPTC2 showed similar hypersensitive to genotoxic agents MMS, HU and CP. (2) The sensitivity of deletion mutants to genotoxic agents: After the strains were treated with HU or MMS for 4 hours, the sur-vival rate of RM1000 strains was about 95% while the survival rate of the deletion mutants declined severely, and there was no obvious difference between these deletion mutants. (3)The filamentous growth of the deletion mutants: After the strains were treated with 0.020% MMS for 4 hours, the wildtype strains showed yeast form while the deletion mutants of CaPPH3, CaPSY2 and the double-gene deletion mutants for CaPPH3 and CaPTC2 showed filamentous form. Then after the MMS was washed out, the wildtype strains generated hypha gradually until the timeout after 6 hours and then generated buds, while the deletion mutants remained filamentous at the same time. The strains were also treated with 40 mM HU for 4 hours and they all showed yeast form. After the HU was washed out, the wildtype strains maitained yeast form until the timeout after 6 hours while the deletion mutants generated hypha gradually. Conclusion:CaPph3 and CaPsy2 play a role in the regulation of the filamentous growth induced by genetic toxic reagents in Candida albicans.
