化学研究
化學研究
화학연구
CHEMICAL RESEARCHES
2013年
6期
595-598,604
,共5页
3-乙基苯并噻唑螺萘并噁嗪%牛血清白蛋白%相互作用%荧光光谱%热力学
3-乙基苯併噻唑螺萘併噁嗪%牛血清白蛋白%相互作用%熒光光譜%熱力學
3-을기분병새서라내병오진%우혈청백단백%상호작용%형광광보%열역학
3-ethyl-benzothiazospironaphthooxazine%bovine serum albumin%interaction%fluo-rescence spectrometry%thermodynamics
采用荧光光谱法和热力学方法研究了3-乙基苯并噻唑螺萘并噁嗪（EBSN ）与牛血清白蛋白（BSA ）的相互作用．结果表明，在pH为7．46的 Tris-HCl及0．01 mol ·L -1的NaCl介质中，EBSN能强烈猝灭BSA的荧光，猝灭机理为形成复合物的静态猝灭．在298、306和313 K时，两者的表观结合常数 Kb分别为1．7620×104，3．3963×104和6．1235×104 L ·mol-1．与此同时，EBSN与BSA的结合反应是自发的，作用力主要为疏水作用力；BSA和EBSN的工作曲线分别为 F0- F ＝2．439c -8．322和（F0- F）/F ＝0．06189c ＋0．5566，对BSA和EBSN的检出限分别为0．015 mg ·L -1和7．61×10-7 mol ·L -1．
採用熒光光譜法和熱力學方法研究瞭3-乙基苯併噻唑螺萘併噁嗪（EBSN ）與牛血清白蛋白（BSA ）的相互作用．結果錶明，在pH為7．46的 Tris-HCl及0．01 mol ·L -1的NaCl介質中，EBSN能彊烈猝滅BSA的熒光，猝滅機理為形成複閤物的靜態猝滅．在298、306和313 K時，兩者的錶觀結閤常數 Kb分彆為1．7620×104，3．3963×104和6．1235×104 L ·mol-1．與此同時，EBSN與BSA的結閤反應是自髮的，作用力主要為疏水作用力；BSA和EBSN的工作麯線分彆為 F0- F ＝2．439c -8．322和（F0- F）/F ＝0．06189c ＋0．5566，對BSA和EBSN的檢齣限分彆為0．015 mg ·L -1和7．61×10-7 mol ·L -1．
채용형광광보법화열역학방법연구료3-을기분병새서라내병오진（EBSN ）여우혈청백단백（BSA ）적상호작용．결과표명，재pH위7．46적 Tris-HCl급0．01 mol ·L -1적NaCl개질중，EBSN능강렬졸멸BSA적형광，졸멸궤리위형성복합물적정태졸멸．재298、306화313 K시，량자적표관결합상수 Kb분별위1．7620×104，3．3963×104화6．1235×104 L ·mol-1．여차동시，EBSN여BSA적결합반응시자발적，작용력주요위소수작용력；BSA화EBSN적공작곡선분별위 F0- F ＝2．439c -8．322화（F0- F）/F ＝0．06189c ＋0．5566，대BSA화EBSN적검출한분별위0．015 mg ·L -1화7．61×10-7 mol ·L -1．
The interaction between 3-ethyl-benzothiazospironaphthooxazine (EBSN) and bovine serum albumin(BSA ) was studied by fluorescence spectrometry and thermodynamics .It was found that in tris-HCl medium (pH = 7 .46) and 0 .01 mol ·L -1 NaCl medium ,EBSN is able to strongly quench the intrinsic fluorescence of BSA through a static quenching mechanism in-volving the formation of a complex .Besides ,EBSN and BSA have binding constants of 1 .762 0 × 104 ,3 .396 3 × 104 ,and 6 .123 5 × 104 L ·mol-1 at 298 ,306 and 313 K ,respectively .In the meantime ,the binding reaction between EBSN and BSA is spontaneous , and hydrophobic forces are predominant in the binding process .Moreover ,the working curves for BSA and EB-SN are established as F0 - F = 2 .439c - 8 .322 and (F0 -F)/F = 0 .061 89c + 0 .556 6 ,re-spectively .The detection limits for BSA and EBSN are determined to be 0 .015 mg ·L -1 and 7 .61 × 10-7 mol ·L -1 ,respectively .
