CAJ | 학술논문

采用荧光光谱法研究了在pH ＝6、不同温度（25、35和45℃）下对磺酸基杯[6]芳烃（SCX6）与葛根素的包合作用；并利用最小二乘法进行非线性拟合，计算出不同温度下的包合常数，初步验证了SCX6与葛根素的包合模式。结果表明，固定葛根素的浓度、逐渐增加对磺酸基杯[6]芳烃的浓度，葛根素荧光强度有规律地降低，表明SCX6和葛根素形成主-客体包合物。在不同温度下，葛根素与SCX6的包合比均为1∶1；且其包合过程可自发进行，温度越高，包合越易发生。
채용형광광보법연구료재pH ＝6、불동온도（25、35화45℃）하대광산기배[6]방경（SCX6）여갈근소적포합작용；병이용최소이승법진행비선성의합，계산출불동온도하적포합상수，초보험증료SCX6여갈근소적포합모식。결과표명，고정갈근소적농도、축점증가대광산기배[6]방경적농도，갈근소형광강도유규률지강저，표명SCX6화갈근소형성주-객체포합물。재불동온도하，갈근소여SCX6적포합비균위1∶1；차기포합과정가자발진행，온도월고，포합월역발생。
The inclusion interaction between p-sulfocalix [6]arene (denoted as SCX6) and puerarin at pH = 6 and different temperatures (25 ,35 and 45 ℃) was studied by means of flu-orescence spectrometry .The inclusion constants at different temperatures were calculated by nonlinear fitting with least square method ,and the inclusion mode was then primarily verified . It was found that ,at a fixed concentration of puerarin ,the fluorescence of puerarin declines regularly and gradually with increasing concentration of SCX 6 ,which demonstrates that SCX6 and puerarin react to form inclusion compound at pH = 6 .Besides ,puerarin and p-sulfocalix [6]arene remain an inclusion ratio of 1∶1 under different temperatures ,and their inclusion process occurs spontaneously .Particularly ,the higher the temperature is ,the easier their in-clusion process is .