农业科学与技术（英文版）
農業科學與技術（英文版）
농업과학여기술（영문판）
AGRICULTURAL SCIENCE & TECHNOLOGY
2013年
10期
1417-1420,1428
,共5页
饶海%周镁%秦拴梅%黄秀平%覃容贵
饒海%週鎂%秦拴梅%黃秀平%覃容貴
요해%주미%진전매%황수평%담용귀
千里光(Climbing Groundsel Herb)%总生物碱%紫外分光光度法%抗菌作用
韆裏光(Climbing Groundsel Herb)%總生物堿%紫外分光光度法%抗菌作用
천리광(Climbing Groundsel Herb)%총생물감%자외분광광도법%항균작용
Climbing groundsel herb (Senecio scandens Buch.-Ham)%Total alkaloids%Ultraviolet spectrophotometry%Antibacterial activity
[目的]比较黔产千里光不同溶剂提取物总生物碱的含量及其抗菌作用。[方法]采用紫外分光光度法测定千里光中总生物碱的含量，并采用牛津杯法观察各提取物的抗菌作用。[结果]采用紫外分光光度法测定千里光中总生物碱浓度在0.0110~0.0550 mg/ml范围内与吸光度的线性关系良好，回归方程为 Y=23.654X+0.021，平均回收率为99.2%，R=0.9999。千里光浓度60%醇提液总生物碱含量为38.71 mg/g，浓度95%醇提液生物碱含量为52.25 mg/g，水煎液总生物碱含量为60.50 mg/g。浓度60%乙醇提取液对肺炎链球菌、金黄色葡萄球菌和大肠杆菌抑制作用较强；水提液对大肠杆菌抑制作用较强；浓度95%乙醇提取液抗菌作用较弱，对绿脓杆菌无抑制作用。[结论]该方法准确、简便、重复性好，可用于黔产千里光中生物碱的含量测定；千里光提取物总生物碱的含量与其抗菌作用无正相关关系。
[目的]比較黔產韆裏光不同溶劑提取物總生物堿的含量及其抗菌作用。[方法]採用紫外分光光度法測定韆裏光中總生物堿的含量，併採用牛津杯法觀察各提取物的抗菌作用。[結果]採用紫外分光光度法測定韆裏光中總生物堿濃度在0.0110~0.0550 mg/ml範圍內與吸光度的線性關繫良好，迴歸方程為 Y=23.654X+0.021，平均迴收率為99.2%，R=0.9999。韆裏光濃度60%醇提液總生物堿含量為38.71 mg/g，濃度95%醇提液生物堿含量為52.25 mg/g，水煎液總生物堿含量為60.50 mg/g。濃度60%乙醇提取液對肺炎鏈毬菌、金黃色葡萄毬菌和大腸桿菌抑製作用較彊；水提液對大腸桿菌抑製作用較彊；濃度95%乙醇提取液抗菌作用較弱，對綠膿桿菌無抑製作用。[結論]該方法準確、簡便、重複性好，可用于黔產韆裏光中生物堿的含量測定；韆裏光提取物總生物堿的含量與其抗菌作用無正相關關繫。
[목적]비교검산천리광불동용제제취물총생물감적함량급기항균작용。[방법]채용자외분광광도법측정천리광중총생물감적함량，병채용우진배법관찰각제취물적항균작용。[결과]채용자외분광광도법측정천리광중총생물감농도재0.0110~0.0550 mg/ml범위내여흡광도적선성관계량호，회귀방정위 Y=23.654X+0.021，평균회수솔위99.2%，R=0.9999。천리광농도60%순제액총생물감함량위38.71 mg/g，농도95%순제액생물감함량위52.25 mg/g，수전액총생물감함량위60.50 mg/g。농도60%을순제취액대폐염련구균、금황색포도구균화대장간균억제작용교강；수제액대대장간균억제작용교강；농도95%을순제취액항균작용교약，대록농간균무억제작용。[결론]해방법준학、간편、중복성호，가용우검산천리광중생물감적함량측정；천리광제취물총생물감적함량여기항균작용무정상관관계。
[Objective] This study aimed to compare the contents of total alkaloids in different solvent extracts from climbing groundsel herb produced in Guizhou Province and their antibacterial activity. [Method] The content of total alkaloids was measured by UV spectrophotometry, and Oxford cups were used to investigate the antibacterial activity of each solvent extract. [Result] There was a good linear correlation between the absorbance measured by the UV spectrophotometer and the content of total al-kaloids within the concentration of 0.011 0-0.054 8 mg/ml at 207 nm, and the re-gression equation was Y=23.654X+0.021, R=0. 999 7 and the average recovery rate was 99.2%. The contents of total alkaloids in 60% ethanol extract, 95% ethanol and water ethanol were 38.71, 52.25 and 60.50 mg/g, respectively. The 60% ethanol ex-tract had strong antibacterial activity against Streptococcus pneumoniae, Staphylo-coccus aureus and Escherichia coli; the water extractive had stronger antibacterial activity against Escherichia coli; 95% ethanol extract had weak antibacterial activity, with no inhibition of Pseudomonas aeruginosa. [Conclusion] This method is accurate, simple, with good repeatability, which can be used for the determination of alkaloids content of the climbing groundsel herb; there is no positive correlation between the content of total alkaloids in different solvent extracts of climbing groundsel herb and their antibacterial activity.
