CAJ | 학술논문

[目的]选育抗生素高产的炭样小单孢菌突变株，以提高炭样小单孢菌 JXNU-1抗生素的产率。[方法]用亚硝酸钠对产抗生素的炭样小单孢菌 JXNU-1单胞子悬浮液进行诱变处理，结合利福平抗性筛选法筛选。[结果]获得1株抗生素产率比出发菌株提高266.05%的抗生素高产突变株 JXNU-1-16-Y65。[结论]研究证实了亚硝酸钠在诱变选育抗生素高产突变株中的应用价值，同时为炭样小单孢菌JXNU-1抗生素的开发应用奠定基础。
[목적]선육항생소고산적탄양소단포균돌변주，이제고탄양소단포균 JXNU-1항생소적산솔。[방법]용아초산납대산항생소적탄양소단포균 JXNU-1단포자현부액진행유변처리，결합리복평항성사선법사선。[결과]획득1주항생소산솔비출발균주제고266.05%적항생소고산돌변주 JXNU-1-16-Y65。[결론]연구증실료아초산납재유변선육항생소고산돌변주중적응용개치，동시위탄양소단포균JXNU-1항생소적개발응용전정기출。
[Objective] The purpose of the study is to breed Micromonospora car-bonacea highly producing antibiotics and then to improve the antibiotic production. [Method] Sodium Nitrite mutagenesis, combined with rifampicin resistance screening, was used in mutation breeding of M. carbonacea highly producing antibiotics from the strain of M. carbonacea JXNU-1. [Result] The overproducing strain JXNU-1-16-Y65 was screened with the production of antibiotics 266.05% more than that of the original strain. [Conclusion] The effectiveness of sodium nitrite mutation in breeding microorganisms highly producing antibiotic was proved, and the study may lay the foundation on further development and application of the antibiotic from M. car-bonacea JXNU-1.