CAJ | 학술논문

[目的]分离并筛选具有抗黑斑病活性的内生真菌。[方法]从陕西省西安、咸阳、宝鸡、渭南4个地点采集各种类型的健康月季品种，从茎和叶组织中分离内生真菌并通过转接2~3次进行纯化，采用改良的打孔法筛选对黑斑病病原菌有拮抗作用的内生真菌。[结果]共分离获得月季内生真菌67株，其中咸阳采集的样品内生菌的定殖率和分离率最高；月季的茎段内生真菌的定殖率和分离率明显高于叶片。从67株内生真菌中筛选出3株具有抗黑斑病活性的内生菌菌株，并且全部来自宝鸡。[结论]该研究为进一步筛选生防真菌的候选菌株及环境友好型真菌生物防治奠定了基础。
[목적]분리병사선구유항흑반병활성적내생진균。[방법]종합서성서안、함양、보계、위남4개지점채집각충류형적건강월계품충，종경화협조직중분리내생진균병통과전접2~3차진행순화，채용개량적타공법사선대흑반병병원균유길항작용적내생진균。[결과]공분리획득월계내생진균67주，기중함양채집적양품내생균적정식솔화분리솔최고；월계적경단내생진균적정식솔화분리솔명현고우협편。종67주내생진균중사선출3주구유항흑반병활성적내생균균주，병차전부래자보계。[결론]해연구위진일보사선생방진균적후선균주급배경우호형진균생물방치전정료기출。
[Objective] This study aimed to isolate and screen black-spot-resistant en-dophytic fungi strains. [Method] Various species of healthy Rosa chinensis were col-lected from Xi’an, Xianyang, Baoji and Weinan City in Shaanxi Province. Endophytic fungi was isolated from stems and leaves, and purified by 2-3 times of inoculation to screen endophytic fungi antagonistic to Marssonina rosae with modified punching method. [Result] Samples col ected from Xianyang exhibited the highest colonization rate and isolation rate; endophytic fungi strains isolated from stems presented the highest colonization rate and isolation rate compared with leaves. A total of 67 en-dophytic fungi strains were isolated from Rosa chinensis, including 3 black spot-re-sistant strains which were al derived from Baoji. [Conclusion] This study laid the foundation for further screening candidate strains of biocontrol fungi and environ-ment-friendly fungal biological control.