CAJ | 학술논문

[目的]研究不同激素种类及浓度对试管苗鳞片分化不定芽的影响。[方法]将Tiber、Rodina和 Constanta试管苗鳞片在含有不同种类和浓度的激素的培养基中，统计三种东方百合在各处理下的不定芽数。[结果]细胞分裂素对东方百合鳞片分化能力的影响在品种间存在差异，其中6-BA 对 Tiber 和Rodina鳞片分化不定芽的作用效果最好，只是在适宜的浓度水平上因品种不同有所差异。KT对 Constanta鳞片分化不定芽的作用效果最好。生长素对3种东方百合试管苗鳞片分化不定芽质量影响差异不明显，只是在分化系数上有所差异。[结论]适合 Tiber、Rodina 和Constanta试管苗鳞片分化不定芽的培养基分别为 MS+0.2 mg/L 6-BA+0.2 mg/L 2，4-D、MS+1.0 mg/L 6-BA+0.2 mg/L IAA 和 MS+1.0 mg/L KT+0.5 mg/L 2，4-D。
[목적]연구불동격소충류급농도대시관묘린편분화불정아적영향。[방법]장Tiber、Rodina화 Constanta시관묘린편재함유불동충류화농도적격소적배양기중，통계삼충동방백합재각처리하적불정아수。[결과]세포분렬소대동방백합린편분화능력적영향재품충간존재차이，기중6-BA 대 Tiber 화Rodina린편분화불정아적작용효과최호，지시재괄의적농도수평상인품충불동유소차이。KT대 Constanta린편분화불정아적작용효과최호。생장소대3충동방백합시관묘린편분화불정아질량영향차이불명현，지시재분화계수상유소차이。[결론]괄합 Tiber、Rodina 화Constanta시관묘린편분화불정아적배양기분별위 MS+0.2 mg/L 6-BA+0.2 mg/L 2，4-D、MS+1.0 mg/L 6-BA+0.2 mg/L IAA 화 MS+1.0 mg/L KT+0.5 mg/L 2，4-D。
[Objective] The purpose of this study is to investigate the effects of differ-ent phytohormones on the adventitious bud differentiation of oriental lily. [Method] The bulb scales of the test-tube plantlets of Tiber, Rodina and Constanta were cul-tured in media supplemented with different cytokinin and auxin at different concen-tration, and then the adventitious buds in each treatment were calculated. [Result] Cytokinins had different influence on the adventitious bud differentiation of the three oriental lily cultivars. Among them, 6-BA had the best effect to induce the adventi-tious bud differentiation from bulb scales of Tiber and Rodina, but there was some difference in the optimal concentration. KT had the best effect to induce the adven-titious bud differentiation of Constanta. The auxins had little influence on the quality of the adventitious bud of the three oriental lily cultivars, but caused some difference in differentiation coefficients. [Conclusion] The most suitable media for the adventi-tious bud differentiation from bulb scales in vitro of Tiber, Rodina and Constanta were MS+0.2 mg/L 6-BA+0.2 mg/L 2,4-D, MS+1.0 mg/L 6-BA+0.2 mg/L IAA, MS+1.0 mg/L KT+0.5 mg/L 2,4-D, respectively.