浙江中医药大学学报
浙江中醫藥大學學報
절강중의약대학학보
JOURNAL OF ZHEJIANG UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
2013年
11期
1271-1274,1288
,共5页
方芳%方剑乔%刘喆%张静若%池细绿
方芳%方劍喬%劉喆%張靜若%池細綠
방방%방검교%류철%장정약%지세록
电针%慢性炎症疼痛%牛肾上腺髓质22肽%受体%背根神经节
電針%慢性炎癥疼痛%牛腎上腺髓質22肽%受體%揹根神經節
전침%만성염증동통%우신상선수질22태%수체%배근신경절
electroacupuncture%chronic inflammatory pain%bovine adrenal medul a 22%receptor%lumbar dorsal root ganglion
[目的]观察电针对完全福氏佐剂（complete freund’s adjuvant ，CFA）致慢性疼痛大鼠患侧腰背根神经节(dorsal root ganglion, DRG)牛肾上腺髓质22肽（BAM22）与其感觉神经元特异性受体（SNSR）、MOR与DOR基因表达的影响。[方法]200±20g雄性SD大鼠30只，随机分成正常对照组、模型对照组、电针组，每组10只，模型对照组、电针组右足CFA造模。电针组从造模24h开始治疗，患侧“足三里”穴，2/100Hz，30min，1次/d，治疗10d，其余2组不予治疗。采用辐射热法检测大鼠缩爪潜伏期观察大鼠患侧热痛敏变化，免疫组化法检测患侧背根神经节BAM22多肽的含量，定量PCR（quantitative polymerase chain reaction, qPCR）法检测患侧背根神经节SNSR、MOR与DOR mRNA的表达。[结果]经过10d的治疗，电针组的痛阈与模型对照组相比，有显著提高（P<0.05）。免疫组化结果显示，电针干预后，患侧背根神经节的BAM22阳性细胞率比模型对照组有显著的提高（P<0.01）。定量PCR结果显示，电针极大地促进了SNSR（P<0.01）、MOR（P<0.01）与DOR mRNA（P<0.01）在患侧DRG中的表达。[结论]电针对CFA大鼠慢性疼痛有良好的干预作用，推测该作用与电针增强患侧腰背根神经节BAM22多肽表达，增强其非阿片受体SNSR与其阿片受体MOR与DOR mRNA表达相关。
[目的]觀察電針對完全福氏佐劑（complete freund’s adjuvant ，CFA）緻慢性疼痛大鼠患側腰揹根神經節(dorsal root ganglion, DRG)牛腎上腺髓質22肽（BAM22）與其感覺神經元特異性受體（SNSR）、MOR與DOR基因錶達的影響。[方法]200±20g雄性SD大鼠30隻，隨機分成正常對照組、模型對照組、電針組，每組10隻，模型對照組、電針組右足CFA造模。電針組從造模24h開始治療，患側“足三裏”穴，2/100Hz，30min，1次/d，治療10d，其餘2組不予治療。採用輻射熱法檢測大鼠縮爪潛伏期觀察大鼠患側熱痛敏變化，免疫組化法檢測患側揹根神經節BAM22多肽的含量，定量PCR（quantitative polymerase chain reaction, qPCR）法檢測患側揹根神經節SNSR、MOR與DOR mRNA的錶達。[結果]經過10d的治療，電針組的痛閾與模型對照組相比，有顯著提高（P<0.05）。免疫組化結果顯示，電針榦預後，患側揹根神經節的BAM22暘性細胞率比模型對照組有顯著的提高（P<0.01）。定量PCR結果顯示，電針極大地促進瞭SNSR（P<0.01）、MOR（P<0.01）與DOR mRNA（P<0.01）在患側DRG中的錶達。[結論]電針對CFA大鼠慢性疼痛有良好的榦預作用，推測該作用與電針增彊患側腰揹根神經節BAM22多肽錶達，增彊其非阿片受體SNSR與其阿片受體MOR與DOR mRNA錶達相關。
[목적]관찰전침대완전복씨좌제（complete freund’s adjuvant ，CFA）치만성동통대서환측요배근신경절(dorsal root ganglion, DRG)우신상선수질22태（BAM22）여기감각신경원특이성수체（SNSR）、MOR여DOR기인표체적영향。[방법]200±20g웅성SD대서30지，수궤분성정상대조조、모형대조조、전침조，매조10지，모형대조조、전침조우족CFA조모。전침조종조모24h개시치료，환측“족삼리”혈，2/100Hz，30min，1차/d，치료10d，기여2조불여치료。채용복사열법검측대서축조잠복기관찰대서환측열통민변화，면역조화법검측환측배근신경절BAM22다태적함량，정량PCR（quantitative polymerase chain reaction, qPCR）법검측환측배근신경절SNSR、MOR여DOR mRNA적표체。[결과]경과10d적치료，전침조적통역여모형대조조상비，유현저제고（P<0.05）。면역조화결과현시，전침간예후，환측배근신경절적BAM22양성세포솔비모형대조조유현저적제고（P<0.01）。정량PCR결과현시，전침겁대지촉진료SNSR（P<0.01）、MOR（P<0.01）여DOR mRNA（P<0.01）재환측DRG중적표체。[결론]전침대CFA대서만성동통유량호적간예작용，추측해작용여전침증강환측요배근신경절BAM22다태표체，증강기비아편수체SNSR여기아편수체MOR여DOR mRNA표체상관。
[Objective] To observe the effects of electroacupuncture(EA) on expression of bovine adrenal medul a 22(BAM22) and gene expression of its senso-ry neuron-speciic receptor(SNSR), MOR and DOR receptors in lumbar dorsal root ganglion(DRG) in rats with complete Freund ’s adjuvant(CFA)-in-duced chronic in ammatory pain.[Methods] Thirty male SD rats were randomly selected as normal group, model group and EA group,with 10 in each . Model group and EA group were made chronic in ammatory pain induced with CFA. Modeled after 24h, the EA group were treated with 2/100Hz EA in control Zusanli(ST36),30 min each time,once per day, for 10 days, while no treatment was given to other groups. The control thermal pain threshold as an index of therapeutic effect was observed by deceting paw with-drawal latency(PWL), and expressions of BAM22 in the affected side DRG by immuno-histochemical staining, gene expressions of SNSR, MOR and DOR in DRG by real-time fluorescence quantitative PCR were detected.[Results] Com-pared with model group, ten-day EA obviously raised the pain threshold of EA group. The ratio of cells in the affected side DRG expressing BAM22 was significantly increased(P<0.01), and the mRNA amount of SNSR MOR, and DOR(P<0.01) of EA group in the affected side DRG were also remark-ably increased. [Conclusions] EA can get analgesia effect on CFA-induced chronic in ammatory pain, and the effect is closely associated with up-regulat-ing the expressions of BAM22 and the mRNA expressions of its receptors:SNSR, MOR and DOR in affected side DRG.
