CAJ | 학술논문

目的：探讨血红素加氧酶1（HO-1）修饰的大鼠骨髓间充质干细胞（BM MSCs）在体外对淋巴细胞的免疫调节作用。方法体外分离培养和鉴定BM MSCs ，用载有HO-1基因的重组腺病毒载体进行感染，形成HO-1/BM MSCs，与大鼠脾淋巴细胞共培养。 MTT法检测淋巴细胞的增殖活性，碘化丙啶染色流式细胞术分析细胞周期的分布情况，ELISA法检测细胞因子含量，流式细胞仪分析T淋巴细胞CD25、CD71的表达。结果 HO-1/BM MSCs参与共培养的淋巴细胞的增殖活性和细胞周期进程均被抑制、促炎因子（ IL-2、TNF-α和IFN-γ）的分泌量降低、抗炎因子（ IL-10）的分泌量升高、T淋巴细胞CD25和CD71的表达降低，与BM MSCs组相比差异有统计学意义。结论 HO-1/BM MSCs较BM MSCs能够更好地发挥对淋巴细胞的免疫调节作用。
목적：탐토혈홍소가양매1（HO-1）수식적대서골수간충질간세포（BM MSCs）재체외대림파세포적면역조절작용。방법체외분리배양화감정BM MSCs ，용재유HO-1기인적중조선병독재체진행감염，형성HO-1/BM MSCs，여대서비림파세포공배양。 MTT법검측림파세포적증식활성，전화병정염색류식세포술분석세포주기적분포정황，ELISA법검측세포인자함량，류식세포의분석T림파세포CD25、CD71적표체。결과 HO-1/BM MSCs삼여공배양적림파세포적증식활성화세포주기진정균피억제、촉염인자（ IL-2、TNF-α화IFN-γ）적분비량강저、항염인자（ IL-10）적분비량승고、T림파세포CD25화CD71적표체강저，여BM MSCs조상비차이유통계학의의。결론 HO-1/BM MSCs교BM MSCs능구경호지발휘대림파세포적면역조절작용。
Objective To investigate the immunomodulatory effects of heme oxygenase-1 ( HO-1) modified rat bone marrow mesenchymal stem cells ( BM-MSCs) on lymphocytes .Methods Rat BM-MSCs were cultured and identified in vitro.HO-1 gene was transduced into BM-MSCs via recombinant adenovirus (rAdV) to construct HO-1/BM MSCs, which were then co-cultured with rat spleen lymphocytes .The cellu-lar activity of lymphocytes was detected by using MTT method .The cell cycle distributions were analyzed by propidium iodide staining and flow cytometry .ELISA was used to detect the level of cytokines in the super-natant of cultured cells .The expression of lymphocyte activation markers , CD25 and CD71, were measured by using fluorescence labeled antibodies and flow cytometry .Results Compared with the control group , the lymphocytes co-cultured with HO-1/BM MSCs showed a suppressed proliferation and cell cycle progression . HO-1/BM MSCs inhibited the secretion of IL-2, TNF-αand IFN-γ, but enhanced the secretion of IL-10 in lymphocytes.Furthermore, the expression of CD25 and CD71 were also down-regulated by HO-1/BM MSCs. Conclusion Compared with BM MSCs , HO-1/BM MSCs show higher immunosuppressive effects on lym-phocytes .