CAJ | 학술논문

目的：探索降钙素对骨质疏松性骨折愈合过程中基质Ⅰ、Ⅱ、Ⅲ型胶原 mRNA表达的影响，阐明降钙素促进骨质疏松性骨折愈合的机制。方法选用96只2个月龄SD雌性大鼠，64只行双侧卵巢切除（ OVX）后，随机平分为降钙素组和对照组，其余32只为假手术组；10周后所有大鼠在右侧桡骨下端制作1 mm骨缺损的骨折愈合模型。降钙素组从骨折术前1 w至术后8 w连续每天皮下注射鲑鱼降钙素2 IU/kg，另2组注射等量生理盐水。采用原位杂交技术分析7 d，14 d，28 d，56 d，4个时间、点降钙素对骨折修复过程中基质Ⅰ、Ⅱ、Ⅲ型胶原mRNA表达的影响。结果降钙素治疗下，骨基质Ⅰ型胶原mRNA表达的量与对照组比较有显著性差异，假手术组＞降钙素组＞对照组；Ⅲ型胶原mRNA的表达变化则与之相反。降钙素组软骨性骨痂向骨性骨痂转换时间较对照组缩短。结论降钙素能影响胶原mRNA的表达，明显促进骨质疏松性骨折的愈合。
목적：탐색강개소대골질소송성골절유합과정중기질Ⅰ、Ⅱ、Ⅲ형효원 mRNA표체적영향，천명강개소촉진골질소송성골절유합적궤제。방법선용96지2개월령SD자성대서，64지행쌍측란소절제（ OVX）후，수궤평분위강개소조화대조조，기여32지위가수술조；10주후소유대서재우측뇨골하단제작1 mm골결손적골절유합모형。강개소조종골절술전1 w지술후8 w련속매천피하주사해어강개소2 IU/kg，령2조주사등량생리염수。채용원위잡교기술분석7 d，14 d，28 d，56 d，4개시간、점강개소대골절수복과정중기질Ⅰ、Ⅱ、Ⅲ형효원mRNA표체적영향。결과강개소치료하，골기질Ⅰ형효원mRNA표체적량여대조조비교유현저성차이，가수술조＞강개소조＞대조조；Ⅲ형효원mRNA적표체변화칙여지상반。강개소조연골성골가향골성골가전환시간교대조조축단。결론강개소능영향효원mRNA적표체，명현촉진골질소송성골절적유합。
Objective To investigate the effect of calcitonin on the mRNA expression of matrix type I , II, and III collagen during the healing process of osteoporotic fractures , and to illuminate the mechanism of calcitonin in promoting the healing of osteoporotic fractures.Methods A total of 96 2-month female SD rats were selected.Of which, 64 rats received bilateral ovariectomy (OVX), and they were randomly divided into calcitonin therapy group and control group .The other 32 rats were divided into sham operation group.Ten weeks later , all the rats made into fracture models with about 1mm width of the bone defect at the end of the right radius . Rats in the calcitonin therapy group received a subcutaneous injection of 2 IU/kg salmon calcitonin once a day from 1 week before the operation to 8 weeks after the operation .Rats in the other 2 groups were injected with same volume of normal saline .The effect of calcitonin on the mRNA expression of type I , II, and III collagen during the healing process of bone fractures was observed using in situ hybridization on the 7th, 14th, 28th, and 56th day.Results With the treatment of calcitonin , the expression of matrix type I collagen mRNA was significantly different to that in control groups , which indicated as sham operation group >calcitonin therapy group >control group.Whereas, the expression of type III collagen mRNA was opposite to that of type I collagen mRNA .The period in calcitonin therapy group transferring from cartilaginous callus into bony callus was shorter than that in control group .Conclusion Calcitonin can affect the expression of collagen RNA and promote the healing process of osteoporotic fractures obviously .