CAJ | 학술논문

目的：考察阿法骨化醇对糖尿病小鼠骨组织的作用。方法使用腹腔注射链脲佐菌素诱导糖尿病模型。 ELISA方法检测血清骨代谢指标。微焦点定量CT检测骨密度、并经三维重建获得微观结构。使用RT-PCR、蛋白印迹分别检测基因、蛋白表达。结果糖尿病小鼠血清B-ALP、TRACP-5b、尿钙水平升高，股骨远端的骨密度降低，骨组织RAS组分AGT、Renin-R、ANG II表达显著上调。阿法骨化醇降低糖尿病小鼠血清TRACP-5b、尿钙水平，提高骨密度，抑制骨组织高RAS活性，显著下调破骨因子CAII、MMP-9表达。结论阿法骨化醇通过抑制糖尿病小鼠骨组织RAS活性而防治糖尿病性骨质疏松症。
목적：고찰아법골화순대당뇨병소서골조직적작용。방법사용복강주사련뇨좌균소유도당뇨병모형。 ELISA방법검측혈청골대사지표。미초점정량CT검측골밀도、병경삼유중건획득미관결구。사용RT-PCR、단백인적분별검측기인、단백표체。결과당뇨병소서혈청B-ALP、TRACP-5b、뇨개수평승고，고골원단적골밀도강저，골조직RAS조분AGT、Renin-R、ANG II표체현저상조。아법골화순강저당뇨병소서혈청TRACP-5b、뇨개수평，제고골밀도，억제골조직고RAS활성，현저하조파골인자CAII、MMP-9표체。결론아법골화순통과억제당뇨병소서골조직RAS활성이방치당뇨병성골질소송증。
Objective To investigate the effect of alfa-calcidol on bone tissue in diabetic mice .Methods The diabetic mouse model was established using intraperitoneal injection of streptozotocin .Serum bone metabolic markers were detected using ELISA .Bone mineral density was detected using micro-CT, and the three-dimensional microstructure was reconstructed .Gene expression was detected using RT-PCR, and protein expression was detected using Western blotting .Results The serum levels of B-ALP and TRACP-5b and the urinary level of calcium increased in diabetic mice .The bone mineral density of the distal femur reduced , while the expression of RAS components, including AGT, Renin-R, and ANG II, increased significantly.Alfa-calcidol could reduce the level of serum TRACP-5b and urine Ca, improve the bone mineral density , inhibit the high activity of renin-angiotensin system (RAS) in bone tissue, and down-regulate the expression of osteoclastic factors , including CAII and MMP-9, in diabetic mice.Conclusion Alfa-calcidol can prevent diabetes-induced osteoporosis by inhibiting the RAS activity in bone tissue in diabetic mice .