CAJ | 학술논문

为研究不同中药复方对大肠杆菌、沙门氏菌2种临床耐药菌株R质粒的消除作用，以三黄汤等4种中药复方水煎剂为质粒消除剂，以临床分离的多重耐药大肠杆菌及沙门氏菌为靶细菌，采用肉汤二倍稀释法和影印培养法进行质粒消除试验。结果显示，4种中药复方对2种临床耐药菌R质粒均有消除作用（消除率0.7％～9.3％），不同中药复方耐药性消除子均丢失2～3个质粒条带（2000～21000 bp），并丢失耐药表型。表明4种中药复方对耐药大肠杆菌和沙门氏菌R质粒均有消除作用。
위연구불동중약복방대대장간균、사문씨균2충림상내약균주R질립적소제작용，이삼황탕등4충중약복방수전제위질립소제제，이림상분리적다중내약대장간균급사문씨균위파세균，채용육탕이배희석법화영인배양법진행질립소제시험。결과현시，4충중약복방대2충림상내약균R질립균유소제작용（소제솔0.7％～9.3％），불동중약복방내약성소제자균주실2～3개질립조대（2000～21000 bp），병주실내약표형。표명4충중약복방대내약대장간균화사문씨균R질립균유소제작용。
To study the plasmid curing in Escherichia coli and Salmonella by different traditional Chinese medical(TCM ) compounds ,the R plasmid curing experiment was performed using 4 dif-ferent Chinese medical decoctions .The multi-resistant Escherichia coli and Salmonella isolates were used as target bacteria .The influence on R plasmid curing was observed by broth doubling dilution and replica plating method .The results showed that the 4 decoctions exhibited resistance curing effects against Escherichia coli and Salmonella ,the curing rates ranging from 0 .7% -9.3% and 2 or 3 plasmids ,2 000-21 000 bp-long ,were lost in different curing strains .The miss-ing patterns of R plasmid occurred as the disappearance of different resistant phenotypes .This in-dicates that the 4 different Chinese medical compounds have R plasmid curing effects against Escherichia coli and Salmonella .