CAJ | 학술논문

为了明确引起河南省烟草低头黑病的病原菌，在襄城、舞阳两县采集表现烟草低头黑病症状的病株，采用组织分离与单孢分离相结合的方法分别获得Ⅰ号和Ⅱ号2个菌株。形态学鉴定结果表明：在PDA培养基上，菌落正面初期呈白色、黄色或灰色等变化，但后期均能产生分生孢子盘、刚毛、分生孢子梗与分生孢子。rDNA ITS序列分析结果表明，菌株Ⅰ号和菌株Ⅱ号属于同一个种，其序列与序列号为DQ286158．1和 HM231266．1的辣椒炭疽菌（Colletotrichum capsici）ITS序列同源性分别均为100％和99％。因此，河南省烟草低头黑病的病原菌可确定为辣椒炭疽菌[Colletotrichum capsici（Syd ．） Butler & Bisby ]。
위료명학인기하남성연초저두흑병적병원균，재양성、무양량현채집표현연초저두흑병증상적병주，채용조직분리여단포분리상결합적방법분별획득Ⅰ호화Ⅱ호2개균주。형태학감정결과표명：재PDA배양기상，균락정면초기정백색、황색혹회색등변화，단후기균능산생분생포자반、강모、분생포자경여분생포자。rDNA ITS서렬분석결과표명，균주Ⅰ호화균주Ⅱ호속우동일개충，기서렬여서렬호위DQ286158．1화 HM231266．1적랄초탄저균（Colletotrichum capsici）ITS서렬동원성분별균위100％화99％。인차，하남성연초저두흑병적병원균가학정위랄초탄저균[Colletotrichum capsici（Syd ．） Butler & Bisby ]。
In order to identify the pathogen causing tobacco black death disease in Henan prov-ince ,two strains were isolated from the disease infected plants in Xiangcheng and Wuyang by the methods of tissue separation and single spore isolation .The pathogens were identified based on their morphological characteristics and ITS sequences .Studies on the pathogens showed that colo-nies were initially white ,yellow or gray on PDA medium ,and they all produced acervuli ,bristles , conidiophores ,and conidia .Two strains were identified as the same species based on their rDNA-ITS ,which displayed 100% and 99% identities with DQ286158 .1 and HM231266 .1 ,respectively . It is confirmed that Colletotrichum capsici(Syd .) Butler & Bisby is the causal organism of tobac-co black death disease in Henan province .