CAJ | 학술논문

用红外光谱内标法（K3[Fe(CN)6]·6H2O为内标物）测定了氯霉素片中氯霉素的含量。实验表明，混合物红外光谱中氯霉素的测量峰（1686.4cm-1）和K3[Fe(CN)6]·6H2O的内标峰（2117.2cm-1）的峰高比（y=hi/hs）与两者质量比（x=mi/ms）有良好的线性关系，其线性回归方程为y=1.3127x+0.5705，相关系数r＝0.9981。据此测得氯霉素片中氯霉素的含量为77.2%，相对标准偏差为1.8%，加标回收率分别为96.8%~103.8%，测定结果与药典规定的紫外分光光度法相近。该方法测量专属性强，无需分离提取，简便、快速、成本低，测定结果准确，特别适合快速批量检测，有应用价值。
용홍외광보내표법（K3[Fe(CN)6]·6H2O위내표물）측정료록매소편중록매소적함량。실험표명，혼합물홍외광보중록매소적측량봉（1686.4cm-1）화K3[Fe(CN)6]·6H2O적내표봉（2117.2cm-1）적봉고비（y=hi/hs）여량자질량비（x=mi/ms）유량호적선성관계，기선성회귀방정위y=1.3127x+0.5705，상관계수r＝0.9981。거차측득록매소편중록매소적함량위77.2%，상대표준편차위1.8%，가표회수솔분별위96.8%~103.8%，측정결과여약전규정적자외분광광도법상근。해방법측량전속성강，무수분리제취，간편、쾌속、성본저，측정결과준학，특별괄합쾌속비량검측，유응용개치。
Potassium ferricyanide was added into chloramphenicol samples as internal reference and the mixtures were measured by Fou-rier transform infrared spectrophotometry (FT-IR). The peak of chloramphenicol at 1686.4 cm-1 was chosen as quantitative peak and the one of potassium ferricyanide at 2117.2 cm-1 was chosen as inside mark quantitative peak. The ratio of two peak’s absorbance (y=hi/hs) had good linear relationship with their mass ratio (x=mi/ms). The linear regression equation was y=1.3127x+0. 5705 with the correlation coef-ficient r=0.9981. The contents of chloramphenicol in the samples of chloramphenicol tablets was 77.2%, the relatively standard deviation was 1.8%, the recoveries of the method was 96.8%~103.8%. This method was highly specific measure, especially for fast batch testing.