CAJ | 학술논문

目的：在7.0 T磁场下利用MR波谱(MRS)技术结合LCModel后处理，获得脑胶质瘤体中心，瘤体实质部分、瘤体周边组织及对侧正常脑组织代谢物绝对浓度、分析不同部位代谢特征。材料与方法以立体定向移植法将C6胶质瘤细胞植入SD鼠脑右侧基底节区，术后7 d置于7.0 T MR扫描仪下行MRS及MRI扫描，获得的MRS原始波谱数据在LCModel软件上量化分析，SPSS软件统计所得代谢物绝对浓度在各部位之间的差异性。结果从肿瘤中心到对侧正常脑组织，可以观测到NAA、tCr浓度呈逐渐上升趋势，除肿瘤周边组织与对侧正常脑组织间NAA、tCr浓度无统计学差异性外，其他各组间这两种物质浓度均有统计学差异性。从肿瘤中心到对侧正常脑组织，Ala浓度则呈逐渐下降趋势，仅肿瘤实体部分与对侧正常脑组织间Ala浓度有统计学差异性。Tau和Ins浓度在肿瘤周边组织内达到最低，且两种代谢物浓度在肿瘤周边脑组织内与其他各部位之间均有统计学差异性。Glx浓度在肿瘤周边脑组织内达到最高，且各组间Glx浓度间均有统计学差异性。结论超高场MRS技术是分析脑胶质瘤不同部位代谢特征的良好检查手段，Tau、Ins和Glx可能对判断脑胶质瘤潜在瘤体边界提供重要的参考信息。
목적：재7.0 T자장하이용MR파보(MRS)기술결합LCModel후처리，획득뇌효질류체중심，류체실질부분、류체주변조직급대측정상뇌조직대사물절대농도、분석불동부위대사특정。재료여방법이입체정향이식법장C6효질류세포식입SD서뇌우측기저절구，술후7 d치우7.0 T MR소묘의하행MRS급MRI소묘，획득적MRS원시파보수거재LCModel연건상양화분석，SPSS연건통계소득대사물절대농도재각부위지간적차이성。결과종종류중심도대측정상뇌조직，가이관측도NAA、tCr농도정축점상승추세，제종류주변조직여대측정상뇌조직간NAA、tCr농도무통계학차이성외，기타각조간저량충물질농도균유통계학차이성。종종류중심도대측정상뇌조직，Ala농도칙정축점하강추세，부종류실체부분여대측정상뇌조직간Ala농도유통계학차이성。Tau화Ins농도재종류주변조직내체도최저，차량충대사물농도재종류주변뇌조직내여기타각부위지간균유통계학차이성。Glx농도재종류주변뇌조직내체도최고，차각조간Glx농도간균유통계학차이성。결론초고장MRS기술시분석뇌효질류불동부위대사특정적량호검사수단，Tau、Ins화Glx가능대판단뇌효질류잠재류체변계제공중요적삼고신식。
Objective:This study aimed to acquire the metabolite concentrations and metabolic characteristic in four different areas (tumor center, tumor soild part, tumor peritumoral area and contralateral white matter) of brain glioma with the combination of MRS and LCModel method. Materials and Methods:The C6 glioma cells were stereotaxically implanted into the right basal ganglia region of SD rats. The rats then underwent MRS at 7.0 T MR scanner. Neuronal metabolites were measured within the tumor center, tumor solid part, adjacent normal-appearing tissue, and contralateral white-matter. All spectra were quantiifed by LCModel, the whole datas were statistical analyzed by SPSS. Results:A gradual increased concentrations of NAA, tCr , and a decreased of Ala\from the tumor center to the contralateral normal white matter were observed. Moreover, the lowest level of Ins and Tau were found in the tumor peripheral area. There were signiifcant differences in Ins and Tau between the tumor peripheral area and other three areas. The highest Glx peaks appeared in the tumor peripheral part. The statistically significant differences in Glx among each region were also found. Conlustions:MRS in ultra high magnetic field is a valuable method to investigate metabolic speciifcity in different areas of glioma. The MRS data of Tau, Ins and Glx may supply additional information about the location of glioma potential border.