CAJ | 학술논문

目的：采用免疫荧光层析法与电化学发光法定量检测降钙素原（PCT ），对其正确度性能进行评价。方法连续检测10 d ，每天取不同浓度的6～7份临床血清样本，其中高值18份，中值22份，低值28份，共计68份样本分别用广州万孚生物技术服务有限公司出品的免疫荧光层析法仪器与配套试剂（实验方法）和罗氏公司的电化学发光法仪器与配套试剂（比较方法）定量检测 PCT ，分析两种方法检测结果的偏倚和相关性，评估两种方法的可比性。结果两种检测方法配对 t 检验，t ＝0．479，单侧 t0．05（67）＝1．668，故 P ＞0．05；直线回归检测，相关系数0．992，P＜0．05，回归方程：Y ＝0．960 X ＋0．257，检测结果具较好一致性，PCT 医学决定水平0．5 ng/mL 和2．0 ng/mL 处的总符合率为97．06％。结论免疫荧光层析法与电化学发光法检测 PCT 结果具高度相关性和一致性，能够满足临床检测的基本要求。
목적：채용면역형광층석법여전화학발광법정량검측강개소원（PCT ），대기정학도성능진행평개。방법련속검측10 d ，매천취불동농도적6～7빈림상혈청양본，기중고치18빈，중치22빈，저치28빈，공계68빈양본분별용엄주만부생물기술복무유한공사출품적면역형광층석법의기여배투시제（실험방법）화라씨공사적전화학발광법의기여배투시제（비교방법）정량검측 PCT ，분석량충방법검측결과적편의화상관성，평고량충방법적가비성。결과량충검측방법배대 t 검험，t ＝0．479，단측 t0．05（67）＝1．668，고 P ＞0．05；직선회귀검측，상관계수0．992，P＜0．05，회귀방정：Y ＝0．960 X ＋0．257，검측결과구교호일치성，PCT 의학결정수평0．5 ng/mL 화2．0 ng/mL 처적총부합솔위97．06％。결론면역형광층석법여전화학발광법검측 PCT 결과구고도상관성화일치성，능구만족림상검측적기본요구。
Objective Using immunofluorescence assay and the electrochemical luminescence method for quan-titative detection of procalcitonin (PCT ) ,to evaluate the accuracy of performance .Methods 10 consecutive days ,6 to 7 clinical serum samples with different concentration of every day ,the high value of 18 ,median 22 ,low value of 28 ,a total of 68 samples were used in Guangzhou Wanfu Biological Technology Service Co immunofluorescence chromatog -raphy instruments and reagents(Experiment method) and electrochemical Roche chemiluminescence instrument and reagents(comparison method) and quantitative detection of PCT ,analysis of both bias and correlation detection re-sults ,two methods of evaluating comparability .Results Two methods for the detection of paired t test ,t = 0 .479 , t0 .05(67) = 1 .668 ,P > 0 .05 ;Linear regression analysis ,r = 0 .992 ,> 0 .975 .The regression equation :Y = 0 .960 X +0 .257 ,the detection results have good consistency ,PCT medical decision the total coincidence rate of 97 .06% levels of 0 .5ng/ml and 2 .0ng/ml .Conclusion Compared with experimental method in the detection of PCT with a high de-gree of correlation and consistency ,to meet the basic requirements of clinical detection .