CAJ | 학술논문

目的：探讨干化学法检测肝素抗凝血浆用于急诊生化检验的可行性。方法采用 Vitros5600全自动干化学生化分析仪分别检测200例急诊患者的肝素抗凝血浆与血清标本的23项急诊常规生化指标，并通过统计学分析及差异分析对检测结果进行比较。结果血清与血浆检测结果比较，23项指标中钾、丙氨酸氨基转移酶（ALT ）、γ-谷氨酰转移酶、总蛋白、清蛋白、肌酐、钙、磷、镁、乳酸脱氢酶、天门冬氨酸氨基转移酶（AST ）、脂肪酶12项检测结果差异有统计学意义；但经临床可接受性分析发现，仅 ALT 、AST 2项血清与血浆检测结果差异为临床不可接受，其余10个项目差异均小于1/2CLIA′88允许误差，其差异为临床可接受。结论肝素抗凝血浆可代替血清用于急诊干化学法检测多项指标，且具有快速分离血浆，无凝块等优点，更符合急诊临床需求，而 ALT 、AST 2项应用肝素抗凝血浆进行干化学检测时需重新建立血浆参考范围。
목적：탐토간화학법검측간소항응혈장용우급진생화검험적가행성。방법채용 Vitros5600전자동간화학생화분석의분별검측200례급진환자적간소항응혈장여혈청표본적23항급진상규생화지표，병통과통계학분석급차이분석대검측결과진행비교。결과혈청여혈장검측결과비교，23항지표중갑、병안산안기전이매（ALT ）、γ-곡안선전이매、총단백、청단백、기항、개、린、미、유산탈경매、천문동안산안기전이매（AST ）、지방매12항검측결과차이유통계학의의；단경림상가접수성분석발현，부 ALT 、AST 2항혈청여혈장검측결과차이위림상불가접수，기여10개항목차이균소우1/2CLIA′88윤허오차，기차이위림상가접수。결론간소항응혈장가대체혈청용우급진간화학법검측다항지표，차구유쾌속분리혈장，무응괴등우점，경부합급진림상수구，이 ALT 、AST 2항응용간소항응혈장진행간화학검측시수중신건립혈장삼고범위。
Objective To investigate the feasibility of the dry chemistry method detecting heparin anticoagu -lant plasma for the emergency biochemical test .Methods 23 routine biochemistry indicators of the heparin anticoagu-lant plasma and that of the sample of serum in 200 emergency patients were each tested by Vitros 5600 automatic dry biochemistry instrument .And then the results were compared though statistics method .Results In the 23 indicators compared ,12 indicators had statistically significant difference ,which includes K ,ALT ,GGT ,TP ,ALB ,CREA ,Ca , PHOS ,Mg ,LDH ,AST and LIPA ,however ,by clinical acceptable analysis ,only 2 indicators′differences were unac-ceptable and the other 10 indicators′differences were less than 1/2 CLIA′88 total error allowance which were accepta-ble by clinic .Conclusion Heparin anticoagulant plasma could be used to replace the sample of serum for many emer-gency dry chemistry tests ,with the advantage of rapid separation of plasma ,without clotting and according with clini-cal demand ,however ,it is necessary to rebuild plasma reference range for AST and ALT .