CAJ | 학술논문

目的：比较水蒸气蒸馏(简称SD)法和超临界CO2萃取法(简称SFE-CO2萃取法)提取复方苦黄方中蛇床子、防风、苍术等三味中药的挥发性成分及相对含量。方法：采用气相色谱-质谱联用( GC-MS)法,对SD法提取的挥发油和SFE-CO2萃取法得到的萃取物进行分析,比较两种提取物中的化学成分,并用峰面积归一化法比较各成分的相对含量。结果： SD法提取的挥发油中分离鉴定出36种成分,SFE-CO2法得到的萃取物中分离鉴定出31种成分,其中22种成分相同,相对分子量主要集中在200~230之间。结论：SFE-CO2法对复方苦黄方中蛇床子、防风、苍术等三味中药有效成分的提取具有更高的选择性,是更适合本方剂的提取方法。
목적：비교수증기증류(간칭SD)법화초림계CO2췌취법(간칭SFE-CO2췌취법)제취복방고황방중사상자、방풍、창술등삼미중약적휘발성성분급상대함량。방법：채용기상색보-질보련용( GC-MS)법,대SD법제취적휘발유화SFE-CO2췌취법득도적췌취물진행분석,비교량충제취물중적화학성분,병용봉면적귀일화법비교각성분적상대함량。결과： SD법제취적휘발유중분리감정출36충성분,SFE-CO2법득도적췌취물중분리감정출31충성분,기중22충성분상동,상대분자량주요집중재200~230지간。결론：SFE-CO2법대복방고황방중사상자、방풍、창술등삼미중약유효성분적제취구유경고적선택성,시경괄합본방제적제취방법。
Objective:To compare the steam distillation( SD) and supercritical CO2 ( SFE-CO2 ) method in the extraction of vola-tile constituents from such 3 traditional Chinese drugs as cnidium monnieri, radix sileris and atractylodes lancea in compound Kuhuang prescription. Methods:Essential oil was extracted from the 3 traditional Chinese drugs by SD and SFE-CO2 method, respectively. The components were identified by GC-MS, and their relative contents were calculated with peak area normalization method. Results:To-tally 36 components were isolated and identified using SD extraction and 31 components were identified using SFE-CO2 extraction, a-mong them, 22 components were the same and their relative molecular weight mainly concentrated within 200-230. Conclusion: SFE-CO2 method can extract effective components from the 3 traditional Chinese drugs in compound Kuhuang prescription with higher selec-tivity, which is the more suitable extraction method for essential oil from the prescription.