CAJ | 학술논문

为了探究 X射线辐射对小鼠肾组织乳酸脱氢酶(LDH)、Na+-K+ATP 酶活性及 Caspase-3蛋白、Bax蛋白表达的影响,对96只成体小鼠用不同剂量的 X射线(0、1、3、5 Gy)进行全身辐射,分别于辐射后5、10、15 d和20 d,用比色法测量小鼠肾组织结构中乳酸脱氢酶、Na+-K+ATP 酶的活性变化,用免疫组化法测量小鼠肾组织结构中Caspase-3蛋白、Bax蛋白表达的变化。结果发现：经 X射线辐射后小鼠肾组织乳酸脱氢酶活性有不同程度增加, Na+-K+ATP酶活性有不同程度的降低,Caspase-3蛋白和 Bax蛋白的表达增强。据此认为,X 射线辐射剂对小鼠肾脏组织细胞有明显的损伤效应。
위료탐구 X사선복사대소서신조직유산탈경매(LDH)、Na+-K+ATP 매활성급 Caspase-3단백、Bax단백표체적영향,대96지성체소서용불동제량적 X사선(0、1、3、5 Gy)진행전신복사,분별우복사후5、10、15 d화20 d,용비색법측량소서신조직결구중유산탈경매、Na+-K+ATP 매적활성변화,용면역조화법측량소서신조직결구중Caspase-3단백、Bax단백표체적변화。결과발현：경 X사선복사후소서신조직유산탈경매활성유불동정도증가, Na+-K+ATP매활성유불동정도적강저,Caspase-3단백화 Bax단백적표체증강。거차인위,X 사선복사제대소서신장조직세포유명현적손상효응。
To explore the effects of the X-ray with different dosages on the activity of lactate dehydro-genase(LDH),Na+-K+ATP enzyme and the expression of Caspase-3 protein and Bax protein of mouse kidney tissue,96 mice(Musmusculus)are irradiated with different dosages (0,1,3,5 Gy)of X-ray.At 5, 10,15,20 d after irradiation,changes of the activity of LDH and Na+-K+ATP enzyme of mouse kidney tis-sue are examined by colorimetric method and the changes of the expression of Caspase-3 protein and Bax protein of mouse kidney tissue are examined by immunohistochemical staining.The result showed the activ-ity of LDH increased to different degrees,but the activity of Na+-K+ATP enzyme significantly decreased, both of the Caspase-3 protein and Bax protein expression level increased.X-ray radiation could cause obvi-ous inj ury to the tissue structure and function of kidney.