CAJ | 학술논문

家蚕类胡萝卜素加氧酶 BmninaB基因是家蚕视觉形成的关键基因之一。实验以大造品系雌蛾卵巢的cDNA为模板，进行RT-PCR扩增，发现在扩增结果中总有一条非特异性的小片段。本研究对该非特异性小片段进行了克隆测序，并对其出现的原因进行了分析，同时也对家蚕类胡萝卜素加氧酶 BmninaB基因的扩增条件进行了探索。
가잠류호라복소가양매 BmninaB기인시가잠시각형성적관건기인지일。실험이대조품계자아란소적cDNA위모판，진행RT-PCR확증，발현재확증결과중총유일조비특이성적소편단。본연구대해비특이성소편단진행료극륭측서，병대기출현적원인진행료분석，동시야대가잠류호라복소가양매 BmninaB기인적확증조건진행료탐색。
Carotenoid oxygenase BmninaB is one of the key genes in visual formation of Bomby x mori .While using RT-PCR to amplify the BmninaB gene with moth ovarian cDNA of Dazao as the template ,we surprisingly discovered that a small non-specific fragment always ap-peared .In this study ,we cloned and sequenced this non-specific fragment and analyzed the reasons for its occurrence .We also explored the optimum conditions for amplifying carote-noid oxygenase BmninaB gene in B .mori .