CAJ | 학술논문

目的：观察核因子-κB( NF-κB)在油酸致大鼠急性肺损伤时肺组织中的表达变化，探讨葛根素对急性肺损伤的保护作用及其可能机制。方法：24只大鼠随机分为3组：对照组( Control)、油酸组( OA)、葛根素预处理组( Pue)。 Control组大鼠尾静脉注射生理盐水0.1 ml·kg-1；OA组大鼠尾静脉注射油酸0.1 ml·kg-1；Pue组大鼠在注射油酸前30 min腹腔注射葛根素30 mg·kg-1。光镜下观察肺组织病理形态学改变，免疫组织化学染色技术观察NF-κB在肺组织中的表达变化。结果：Pue组较OA组肺组织损伤情况明显减轻。 OA组肺组织NF-κB的阳性表达(0.39±0.07)与Conrtol组(0.12±0.04)相比显著增加(P<0.05)；应用葛根素后肺组织NF-κB的阳性表达(0.24±0.05)明显减少，但仍高于Conrtol组(P<0.05)。结论：葛根素可能通过抑制NF-κB的表达减轻急性肺损伤时肺组织的炎症反应。
목적：관찰핵인자-κB( NF-κB)재유산치대서급성폐손상시폐조직중적표체변화，탐토갈근소대급성폐손상적보호작용급기가능궤제。방법：24지대서수궤분위3조：대조조( Control)、유산조( OA)、갈근소예처리조( Pue)。 Control조대서미정맥주사생리염수0.1 ml·kg-1；OA조대서미정맥주사유산0.1 ml·kg-1；Pue조대서재주사유산전30 min복강주사갈근소30 mg·kg-1。광경하관찰폐조직병리형태학개변，면역조직화학염색기술관찰NF-κB재폐조직중적표체변화。결과：Pue조교OA조폐조직손상정황명현감경。 OA조폐조직NF-κB적양성표체(0.39±0.07)여Conrtol조(0.12±0.04)상비현저증가(P<0.05)；응용갈근소후폐조직NF-κB적양성표체(0.24±0.05)명현감소，단잉고우Conrtol조(P<0.05)。결론：갈근소가능통과억제NF-κB적표체감경급성폐손상시폐조직적염증반응。
Objective:To observe the expression of nuclear factor-kappa B( NF-κB) in rats with acute lung injury ( ALI) caused by oleic acid ( OA) and investigate the protective effect of puerarin ( Pue) in ALI. Methods:Twenty-four rats were randomly divided into three groups:the control group, OA group and Pue group. Morphological changes in lung tissues were observed under a light mi-croscope and the expression of NF-κB in lung tissues were examined. Results:Morphological changes in lung tissues showed that pul-monary inflammation was relieved in Pue group compared with that in OA group. Compared with the control group, the expression of NF-κB was increased obviously in OA group (0. 39 ± 0. 07 vs 0. 12 ± 0. 04, P<0. 05), and that was decreased obviously in Pue group (0. 24 ± 0. 05), which was still higher than that in the control group (P<0. 05). Conclusion:Puerarin can alleviate the inflammation reaction in ALI through inhibiting the expression of NF-κB.