CAJ | 학술논문

目的探索应用不同的离心速度来制备的富血小板血浆（platelet rich plasma，PRP）中TGF生长因子的含量，并做相关分析，尝试阐明制备PRP最合适的离心次数及速度。方法采集自愿者外周血200ml，应用EDTA抗凝，4o保存以备用。应用全自动血液分析仪进行血小板计数，采用二次离心法（不同的离心速度、时间）分离出PRP，再次用全自动血液分析仪进行血小板计数，然后用ELISA法检测所得的PRP中的TGF-β的浓度。结果外周血中的血小板浓度为146.36×109L，PRP的血小板浓度为(757.27±55.07)×109L。 PRP中的TGF-β浓度为(737.68±62.7)ng/ml。其中以900g，10min，700g，10min，这个离心速度与时间组合所得的PRP血小板浓度及TGF-β浓度最高（881.5×109L，824.2 ng/ml）。结论 PRP的制备的质量与离心速度，离心时间密切相关，合适的离心速度，时间可以得到高浓度并富含生长因子的PRP。
목적탐색응용불동적리심속도래제비적부혈소판혈장（platelet rich plasma，PRP）중TGF생장인자적함량，병주상관분석，상시천명제비PRP최합괄적리심차수급속도。방법채집자원자외주혈200ml，응용EDTA항응，4o보존이비용。응용전자동혈액분석의진행혈소판계수，채용이차리심법（불동적리심속도、시간）분리출PRP，재차용전자동혈액분석의진행혈소판계수，연후용ELISA법검측소득적PRP중적TGF-β적농도。결과외주혈중적혈소판농도위146.36×109L，PRP적혈소판농도위(757.27±55.07)×109L。 PRP중적TGF-β농도위(737.68±62.7)ng/ml。기중이900g，10min，700g，10min，저개리심속도여시간조합소득적PRP혈소판농도급TGF-β농도최고（881.5×109L，824.2 ng/ml）。결론 PRP적제비적질량여리심속도，리심시간밀절상관，합괄적리심속도，시간가이득도고농도병부함생장인자적PRP。
Objective To detect the concentration of TGF-β in platelet rich plasma (PRP) that was prepared under dif erent centrifugal force and time, and make a related analysis. Aiming to investigate the most appropriate centrifugal force and time for preparing PRP. Methods Collect 200ml peripheral blood from volunteers, anticoagulated it with EDTA at stored at 4℃ for later use. Ful-automatic blood analyzer was used for the platelet counting, isolated the PRP by quadratic centrifugation (with dif erent centrifugal force and time),then counted the platelet again with full-automatic blood analyzer. The concentration of TGF-β in PRP was detected by ELISA detection. Results The concentration of platelet in peripheral blood was 146.36í109/L, and in PRP was (757.27±55.07)í109L.The concentration of TGF-β in PRP was (737.68±62.7)ng/ml.We found that the concentration of TGF-β in PRP that isolated under 900g,10min combined with 700g,10min was the highest (881.5í10)9/L,824.2 ng/ml).Conclusion The quality of PRP the preparation is closely related to the centrifugal force and time. The appropriate centrifugation and time is more likely to a get high concentration of PRP that rich in growth factors.