CAJ | 학술논문

目的：观察甲状旁腺激素与雌激素单独和联合应用对去势雌性大鼠牙槽骨代谢的影响。方法选用4月龄雌性Wistar大鼠66只，分成两组，分别为伪手术组（n=18）和去势组（n=48）。8周后各处死8只证实骨质疏松造模成功。剩余的伪手术组（A组，n=10）Wistar大鼠作为对照；将剩余的去势组Wistar大鼠随机分成4组，分别为去势组（B组）、去势加雌激素组（C组）、去势加甲状旁腺激素组（D组）、去势加雌激素和甲状旁腺激素组（E组），每组10只。A组和B组注射生理盐水（1mL·kg-1）；C组注射苯甲酸雌二醇（10μg·kg-1）；D组注射甲状旁腺激素（20μg·kg-1）；E组注射苯甲酸雌二醇（10μg·kg-1）和甲状旁腺激素（20μg·kg-1）。隔日腹腔注射1次，用药8周。治疗后处死大鼠并测定牙槽骨骨密度，观察每组大鼠骨组织形态计量学参数及血清钙、磷、碱性磷酸酶（ALP）水平。结果去势手术8周后，去势组大鼠牙槽骨密度明显低于伪手术组（P<0.05）。用药8周后，C、D、E组骨密度、骨小梁面积百分比（%Tb.Ar）、骨小梁宽度（Tb.Th）和骨小梁数量（Tb.N）较B组均有明显提高，其中E组提高最明显（P<0.05）。各组血清钙磷值无明显改变（P>0.05），B组ALP值较A组明显升高（P<0.05）。结论间歇性、小剂量注射甲状旁腺激素能增加去势大鼠牙槽骨的骨密度和改善骨结构，与雌激素联合使用对骨质疏松的治疗有协同作用。
목적：관찰갑상방선격소여자격소단독화연합응용대거세자성대서아조골대사적영향。방법선용4월령자성Wistar대서66지，분성량조，분별위위수술조（n=18）화거세조（n=48）。8주후각처사8지증실골질소송조모성공。잉여적위수술조（A조，n=10）Wistar대서작위대조；장잉여적거세조Wistar대서수궤분성4조，분별위거세조（B조）、거세가자격소조（C조）、거세가갑상방선격소조（D조）、거세가자격소화갑상방선격소조（E조），매조10지。A조화B조주사생리염수（1mL·kg-1）；C조주사분갑산자이순（10μg·kg-1）；D조주사갑상방선격소（20μg·kg-1）；E조주사분갑산자이순（10μg·kg-1）화갑상방선격소（20μg·kg-1）。격일복강주사1차，용약8주。치료후처사대서병측정아조골골밀도，관찰매조대서골조직형태계량학삼수급혈청개、린、감성린산매（ALP）수평。결과거세수술8주후，거세조대서아조골밀도명현저우위수술조（P<0.05）。용약8주후，C、D、E조골밀도、골소량면적백분비（%Tb.Ar）、골소량관도（Tb.Th）화골소량수량（Tb.N）교B조균유명현제고，기중E조제고최명현（P<0.05）。각조혈청개린치무명현개변（P>0.05），B조ALP치교A조명현승고（P<0.05）。결론간헐성、소제량주사갑상방선격소능증가거세대서아조골적골밀도화개선골결구，여자격소연합사용대골질소송적치료유협동작용。
Objective To investigate the influence of parathyroid hormone and estrogen on alveolar bone metabolism of castrated female rats. Methods Sixty-six female Wistar rats which were healthy and 4 months old were divided into two groups, with group SHAM (n=18) and group ovariectomy (OVX) (n=48). After 8 weeks of ovariectomy, the osteoporosis model was confirmed by examing 8 ovariectomized and sham-operated rats. The rest 10 rats in group SHAM were the control group (group A). The rest 40 rats in group OVX were divided into ovariectomized group (group B), ovariectomized and treated with estrogen (group C), ovariectomized and treated with parathyroid hormone (group D), ovariectomized and treated with estrogen and parathyroid hormone (group E) at random with 10 in each group. Group A and B injected physiological saline (1 mL·kg-1), group C injected estradiol benzoate (10 μg·kg-1), group D injected parathyroid hormone (20 μg·kg-1), group E injected parathyroid hormone (20 μg·kg-1) and estradiol benzoate (10 μg·kg-1). The intraperitoneal injection were maken every other day to rats in each group, which continued for 8 weeks. The bone mineral density (BMD), bone histomorphology and serum Ca, P, alkaline phosphatase (ALP) were measured after therapy. Results After 8 weeks of ovariectomy, the lumbar BMD of ovariectomized rats were significantly declined compared with those of the sham-operated rats (P<0.05). Eight weeks later after the drug use, the BMD, %Tb.Ar, Tb.Th, Tb.N in group C, D, E were slightly elevated compared to group B, espe-cially the group E (P<0.05). Serum calcium and phosphorus values did not change significantly (P>0.05). ALP values in group B was significantly higher than that in group A (P<0.05). Conclusion Intermittent application of parathyroid hormone in small doses can increase alveolar BMD of castration rats and improve their bone structure. And it can have synergy effects on the treatment of osteoporosis if it is used combining with estrogen.