CAJ | 학술논문

为了解鸡群感染禽坦布苏病毒的情况，应用已建立的间接酶联免疫吸附试验（间接ELISA ）对2010年12月至2013年9月近3年内采自福建、江西、浙江、广东4省发生产蛋异常鸡场的开产海兰蛋鸡、后备海兰蛋鸡2300份血清样品进行禽坦布苏病毒抗体的检测。结果显示，开产蛋鸡血清样品的禽坦布苏病毒抗体阳性率为43.4％，而后备蛋鸡为19.9％；在开产蛋鸡中，以福建省的阳性率最高，为60.7％，而在后备蛋鸡中则以广东省最高，为37.2％。4省采集的蛋鸡血清样品中均检出禽坦布苏病毒抗体，且开产蛋鸡的阳性率高于后备蛋鸡，揭示福建、江西、浙江、广东4省海兰蛋鸡在不同程度上感染禽坦布苏病毒，研究结果可为以上地区乃至我国鸡群做好禽坦布苏病毒感染的预防提供依据。
위료해계군감염금탄포소병독적정황，응용이건립적간접매련면역흡부시험（간접ELISA ）대2010년12월지2013년9월근3년내채자복건、강서、절강、엄동4성발생산단이상계장적개산해란단계、후비해란단계2300빈혈청양품진행금탄포소병독항체적검측。결과현시，개산단계혈청양품적금탄포소병독항체양성솔위43.4％，이후비단계위19.9％；재개산단계중，이복건성적양성솔최고，위60.7％，이재후비단계중칙이광동성최고，위37.2％。4성채집적단계혈청양품중균검출금탄포소병독항체，차개산단계적양성솔고우후비단계，게시복건、강서、절강、엄동4성해란단계재불동정도상감염금탄포소병독，연구결과가위이상지구내지아국계군주호금탄포소병독감염적예방제공의거。
To investigate avian Tembusu virus infection in chickens ,2 300 sera samples were collected from highland laying hens and reserve hens in Fujian ,Jiangxi ,Zhejiang and Guangdong provinces during Dec .2010 to Sep .2013.The antibodies were detected by indirect enzyme-linked immuosorbent assay (ELISA) .The results showed that the samples of laying hens had higher seropositive rate (43.4% ) than that of reserve hens (19.9% ) .For laying hens , specimens from Fujian province had the highest seropositivity (60.7% ) ,and for reserve hens the specimens from Guangdong province had the highest seropositivity (37.2% ) .The antibodies for avian Tembusu virus were detected from serum samples in all four provinces with different antibody positive rate ,and the samples of laying hens had higher seropositive rate than reserve hens .The serological investigation indicated that different degrees of avian Tembusu virus infection existed in all these regions ,providing the scientific basis for preventing avian tembusu virus infection in chickens in these regions and even in China .