CAJ | 학술논문

植物乳杆菌中lai基因编码亚油酸异构酶可催化亚油酸转化生成共轭亚油酸，为了进一步研究lai基因的功能，利用同源重组基因敲除技术，成功构建用于敲除植物乳杆菌lai基因的同源重组敲除载体。这不仅为乳杆菌基因缺失工程菌的构建提供一个快速有效的手段，也为乳杆菌基因功能研究奠定基础。
식물유간균중lai기인편마아유산이구매가최화아유산전화생성공액아유산，위료진일보연구lai기인적공능，이용동원중조기인고제기술，성공구건용우고제식물유간균lai기인적동원중조고제재체。저불부위유간균기인결실공정균적구건제공일개쾌속유효적수단，야위유간균기인공능연구전정기출。
Linoleic acid isomerase can be encoded by the lai gene in Lactobacillus plantarum, the enzyme can catalyzes linoleic acid into conjugated linoleic acid. In order to study the function of gene lai, a homologous recombination knockout vector is successfully constructed by gene knockout technology to knockout lai in Lactobacillus in this study. The successful construction of knockout vectors not only provides a fast and effective means for building Lactobacillus gene deletion engineering bacteria, but also lay the foundation for the study of gene function in Lactobacillus.