仲恺农业工程学院学报
仲愷農業工程學院學報
중개농업공정학원학보
JOURNAL OF ZHONGKAI UNIVERSITY OF AGRICULTURE AND TECHNOLOGY
2012年
4期
31-33,37
,共4页
吴国杰%柳菲%陈嘉恒%张永锷%郑里焕
吳國傑%柳菲%陳嘉恆%張永鍔%鄭裏煥
오국걸%류비%진가항%장영악%정리환
纤维素酶%酶活力%微晶纤维素%水解%还原糖
纖維素酶%酶活力%微晶纖維素%水解%還原糖
섬유소매%매활력%미정섬유소%수해%환원당
cellulase%enzyme activity%microcrystalline cellulose%hydrolysis%reducing sugars
对影响纤维素酶活力的主要因素进行了研究，同时对微晶纤维素水解工艺进行了优化．结果表明，纤维素酶的最适温度为50℃，最适pH为4．8，纤维素酶活力随着反应时间延长和酶液稀释度的增大而提高；最佳微晶纤维素酶水解工艺为：50℃、pH4．8、m（微晶纤维素）：m（纤维素酶）=1：0．12、振荡反应12h，此时水解液中还原糖的质量浓度可达2．398mg／mL．
對影響纖維素酶活力的主要因素進行瞭研究，同時對微晶纖維素水解工藝進行瞭優化．結果錶明，纖維素酶的最適溫度為50℃，最適pH為4．8，纖維素酶活力隨著反應時間延長和酶液稀釋度的增大而提高；最佳微晶纖維素酶水解工藝為：50℃、pH4．8、m（微晶纖維素）：m（纖維素酶）=1：0．12、振盪反應12h，此時水解液中還原糖的質量濃度可達2．398mg／mL．
대영향섬유소매활력적주요인소진행료연구，동시대미정섬유소수해공예진행료우화．결과표명，섬유소매적최괄온도위50℃，최괄pH위4．8，섬유소매활력수착반응시간연장화매액희석도적증대이제고；최가미정섬유소매수해공예위：50℃、pH4．8、m（미정섬유소）：m（섬유소매）=1：0．12、진탕반응12h，차시수해액중환원당적질량농도가체2．398mg／mL．
The main influence factors of cellulase activity were studied, and the hydrolysis conditions of microcrystalline cellulose were optimized. The experimental results showed that the cellulase optimum tem- perature and pH were 50 ℃ and 4. 8, respectively. The cellulase activity was improved with the increase of reaction time and enzyme dilution degree. The mass concentration of reducing sugars was up to 2. 398 mg/mL, under the optimized hydrolysis conditions with 50 ℃ , pH 4. 8, m (microcrystalline cellulose) :m (cellu- lase) = 1: 0. 12, and oscillation reacting 12 h.
