淮阴师范学院学报：自然科学版
淮陰師範學院學報：自然科學版
회음사범학원학보：자연과학판
Journal of Huaiyin Teachers College(Natrual Science Edition)
2012年
3期
261-265
,共5页
鄢贵龙%纪丽莲%罗玉明%胡永红
鄢貴龍%紀麗蓮%囉玉明%鬍永紅
언귀룡%기려련%라옥명%호영홍
野马追%高速逆流色谱%倍半萜烯内酯%分离
野馬追%高速逆流色譜%倍半萜烯內酯%分離
야마추%고속역류색보%배반첩희내지%분리
eupatorium lindleyanum DC%high-speed counter-current chromatography%sesquiterpenoid lactone%separation
建立了应用高速逆流色谱（HSCCC）从野马追乙酸乙酯萃取物中分离纯化3种倍半萜烯内酯的方法．采用正己烷-乙酸乙酯-甲醇-水（1：4：2：3）作为两相溶剂体系，从900mg野马追乙酸乙酯萃取物中分离得到313．Hydroxy-8β-[4＇-hydroxytigloyloxy]-costunolide6．5mg，野马追内酯A28．5mg和野马追内酯B29．3mg，其纯度分别为92．5％，91．7％和93．9％．3种化合物的结构通过ESI-MS和1HNMR进行了鉴定．
建立瞭應用高速逆流色譜（HSCCC）從野馬追乙痠乙酯萃取物中分離純化3種倍半萜烯內酯的方法．採用正己烷-乙痠乙酯-甲醇-水（1：4：2：3）作為兩相溶劑體繫，從900mg野馬追乙痠乙酯萃取物中分離得到313．Hydroxy-8β-[4＇-hydroxytigloyloxy]-costunolide6．5mg，野馬追內酯A28．5mg和野馬追內酯B29．3mg，其純度分彆為92．5％，91．7％和93．9％．3種化閤物的結構通過ESI-MS和1HNMR進行瞭鑒定．
건립료응용고속역류색보（HSCCC）종야마추을산을지췌취물중분리순화3충배반첩희내지적방법．채용정기완-을산을지-갑순-수（1：4：2：3）작위량상용제체계，종900mg야마추을산을지췌취물중분리득도313．Hydroxy-8β-[4＇-hydroxytigloyloxy]-costunolide6．5mg，야마추내지A28．5mg화야마추내지B29．3mg，기순도분별위92．5％，91．7％화93．9％．3충화합물적결구통과ESI-MS화1HNMR진행료감정．
A high-speed counter-current chromatography(HSCCC) method was established for the separation of three sesquiterpenoid lactones from Eupatorium lindleyanum DC. n-butanol fraction. The two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (1:4:2:3) was selected. From 900 mg of n-butanol fraction of Eupatorium lindleyanum DC, 6.5mg of 3β-Hydroxy-8β- [4'-hydroxytigloyloxy ]-costunolide, 28.5 mg of eupalinolide A and 29.3 mg of eupalinolide B were obtained in one-step HSCCC separation, with the purity of 92.5%, 91.7% and 93.9%, respectively, as determined by HPLC. Their structures were further identified by ESI-MS and 1H NMR.
