CAJ | 학술논문

通过 RT－PCR 方法从山西省不同地区养鸡场病、死鸡法氏囊组织中扩增得到 IBDV SX /12 VP2基因全长，将其克隆至 pMD18－T 载体。测序分析结果表明，IBDV SX/12 VP2全长为1356 bp，推导其编码452个氨基酸；核苷酸与氨基酸同源性分析显示，二者均与超强参考毒株同源性较高，分别为99．2％和99．6％；遗传进化树分析结果表明，IB－DV SX/12与超强参考毒株位于同一进化分支，IBDV SX/12高变区关键氨基酸具有以下特征：222S，249Q，254G，256 I，279D，284A，294I，299S 以及 SWSASGS 七肽区不变，超强参考毒株高变区关键氨基酸为：222A，249Q，254G，256I，279D，284A，294I，299S，除222S 外，其他关键位点氨基酸均符合超强毒株特征，因此，从分子水平推断 IBDV SX/12属于超强毒株。
통과 RT－PCR 방법종산서성불동지구양계장병、사계법씨낭조직중확증득도 IBDV SX /12 VP2기인전장，장기극륭지 pMD18－T 재체。측서분석결과표명，IBDV SX/12 VP2전장위1356 bp，추도기편마452개안기산；핵감산여안기산동원성분석현시，이자균여초강삼고독주동원성교고，분별위99．2％화99．6％；유전진화수분석결과표명，IB－DV SX/12여초강삼고독주위우동일진화분지，IBDV SX/12고변구관건안기산구유이하특정：222S，249Q，254G，256 I，279D，284A，294I，299S 이급 SWSASGS 칠태구불변，초강삼고독주고변구관건안기산위：222A，249Q，254G，256I，279D，284A，294I，299S，제222S 외，기타관건위점안기산균부합초강독주특정，인차，종분자수평추단 IBDV SX/12속우초강독주。
The infectious bursal disease virus VP2 gene was amplified from bursa tissues of infected chickens in different areas of Shanxi province by RT -PCR,and then cloned to pMD18-T vector for sequence analysis .The results revealed that the SX /12 VP2 gene contained 1 356 nucleotides and encoded 452 amino acid.Compared with those other stains from GenBank,the SX/12 VP2 shared 99.2% homology with other very virulent infectious bursal dis -ease virus(vvIBDV) strains at nucleotide level and 99.6% homology at amino acid level.The phylogenetic analysis showed that SX /12 was in the same lineage with the vvIBDV reference strains .The key amino acid in hypervariable region except 222(S) represented the typical feature of those of vvIBDV strains at the positions of 249(Q),254 (G),256(I),279(D),284(A),294(I),299(S) and SWSASGS at positions of 326 -332.These results demon-strated that SX /12 was classified to be vvIBDV.