CAJ | 학술논문

为了开发工业用酶，在前期菌株筛选的基础上，利用Design—Expert软件，通过Plackett—Bruman设计与响应面法（RSM）相结合的实验统计方法实现对Serratiaproteamaculans sp．L3菌株产冷β-半乳糖苷酶培养基成分的优化．Plackett—Burman设计筛选出3个显著影响因子：K2HPO4，MgS04和NaCl,应用中心组合设计和响应面分析确定产酶的最优组合为：K2HPO4 0．15g·L^-1，MgSO4 0．50g·L^-1，NaCl 0．76g·L^-1，此时预测的最高A420为0．3704．经过验证实验，结果表示最佳产酶培养基成分条件下，优化后降解ONPG的能力提高了1．1倍．本研究为微生物β-半乳糖苷酶的后续工业化应用提供理论依据．
위료개발공업용매，재전기균주사선적기출상，이용Design—Expert연건，통과Plackett—Bruman설계여향응면법（RSM）상결합적실험통계방법실현대Serratiaproteamaculans sp．L3균주산랭β-반유당감매배양기성분적우화．Plackett—Burman설계사선출3개현저영향인자：K2HPO4，MgS04화NaCl,응용중심조합설계화향응면분석학정산매적최우조합위：K2HPO4 0．15g·L^-1，MgSO4 0．50g·L^-1，NaCl 0．76g·L^-1，차시예측적최고A420위0．3704．경과험증실험，결과표시최가산매배양기성분조건하，우화후강해ONPG적능력제고료1．1배．본연구위미생물β-반유당감매적후속공업화응용제공이론의거．
Using the principles of capacitors connected in series and the method of electrical energy, the ca- pacitances of spherical capacitor filled with linear anisotropy dielectric, logarithmic composite function anisotropy dielectric, and exponential - composite function anisotropy dielectric are calculated. The results show that the capacitances of the three different types are all linear and the proportions are associated with R1,, R2 , which are the radius of the spherical capacitor, and k is linked with the nature of the spherical capacitor.