滁州学院学报
滁州學院學報
저주학원학보
JOURNAL OF CHUZHOU UNIVERSITY
2012年
5期
53-56
,共4页
明党参多糖%明党参可溶性还原糖%展开剂%薄层层析法
明黨參多糖%明黨參可溶性還原糖%展開劑%薄層層析法
명당삼다당%명당삼가용성환원당%전개제%박층층석법
soluble polysaccharides of Changium smyrnioides%reductive polysaccharides of Changium smyrnioides%developing solvents%thin--layer chromatography(TCL)
分别采用苯酚-硫酸法测定了采自滁州市琅琊山的明党参根及茎叶、药房购买的明党参和参照物党参中可溶性总糖含量、还原性多糖含量;利用薄层层析法定性分析了琅琊山明党参和药房购买的明党参多糖组成。结果表明,琅琊山明党参根中的可溶性总糖含量为4.07%,还原性多糖含量为4.58%。而药房购买的明党参中可溶性总糖含量达到了8.18%,还原性多糖含量为9.24%。利用优化后的展开剂正丁醇-异丙醇-乙酸-水(7:5:2:2)展层测定出琅琊山明党参根的样品中除了半乳糖外应该还含有分子量大于半乳糖的未知的糖,而购买的样品中除了麦芽糖和半乳糖外还含有分子量大于半乳糖的糖类。
分彆採用苯酚-硫痠法測定瞭採自滁州市瑯琊山的明黨參根及莖葉、藥房購買的明黨參和參照物黨參中可溶性總糖含量、還原性多糖含量;利用薄層層析法定性分析瞭瑯琊山明黨參和藥房購買的明黨參多糖組成。結果錶明,瑯琊山明黨參根中的可溶性總糖含量為4.07%,還原性多糖含量為4.58%。而藥房購買的明黨參中可溶性總糖含量達到瞭8.18%,還原性多糖含量為9.24%。利用優化後的展開劑正丁醇-異丙醇-乙痠-水(7:5:2:2)展層測定齣瑯琊山明黨參根的樣品中除瞭半乳糖外應該還含有分子量大于半乳糖的未知的糖,而購買的樣品中除瞭麥芽糖和半乳糖外還含有分子量大于半乳糖的糖類。
분별채용분분-류산법측정료채자저주시랑야산적명당삼근급경협、약방구매적명당삼화삼조물당삼중가용성총당함량、환원성다당함량;이용박층층석법정성분석료랑야산명당삼화약방구매적명당삼다당조성。결과표명,랑야산명당삼근중적가용성총당함량위4.07%,환원성다당함량위4.58%。이약방구매적명당삼중가용성총당함량체도료8.18%,환원성다당함량위9.24%。이용우화후적전개제정정순-이병순-을산-수(7:5:2:2)전층측정출랑야산명당삼근적양품중제료반유당외응해환함유분자량대우반유당적미지적당,이구매적양품중제료맥아당화반유당외환함유분자량대우반유당적당류。
Phenol hydrate--sulfuric was used to determine the soluble polysaccharide and reductive poly- saccharides in the root and stem of Changium smyrnioide collected from the Langya mountains in chuzhou and Changium smyrnioides and Radix Codonopsis bought from pharmacy. By using thin chromatography to analyse polysaccharide component quantitative in the Langya mountains and Changium smyrnioides from pharmacy, the results showed that the content of soluble polysaccharides of Changium smyrnioides from Langya mountains is 3. 075 %, the content of reductive polysaccharide is 4. 585 0%. The same content of the Changium smyrnioides from pharmacy respectively is 8. 18% and 9. 24%. Quantitative determination results showed that the polysaccharides mainly composed of the polysaccharide maltose, half lactose and some unknown sugar exist in Changium Smyrnioides from Langya mountains and the ones bought from pharmacy.