河池学院学报
河池學院學報
하지학원학보
JOURNAL OF HECHI UNIVERSITY
2012年
5期
1-8
,共8页
覃勇荣%李秋明%黄振球%余美君%陈贵常%刘旭辉
覃勇榮%李鞦明%黃振毬%餘美君%陳貴常%劉旭輝
담용영%리추명%황진구%여미군%진귀상%류욱휘
桂西北%岩溶地区%鞘花寄生%不同器官%总黄酮含量
桂西北%巖溶地區%鞘花寄生%不同器官%總黃酮含量
계서북%암용지구%초화기생%불동기관%총황동함량
northwest of Guangxi%karst area%Macrosolen cochinchinensis%different organs%flavonoids content
为了开发利用桂西北岩溶地区丰富的药用植物资源,利用超声波协同提取和正交试验,优化鞘花寄生总黄酮提取的工艺条件。利用分光光度法,对阴香、香樟、桂花等7种寄主的鞘花寄生枝叶、花及果实总黄酮含量进行测定。结果表明:(1)鞘花寄生果实总黄酮提取的优化工艺条件为:乙醇浓度30%,超声波提取时间60min,超声波提取功率180W,料液比1:30。在此条件下得到的鞘花寄生果实总黄酮提取率为3.96%。(2)不同寄主的鞘花寄生,其总黄酮含量有明显差异;3n同寄主的鞘花寄生,其不同器官的总黄酮含量也有极显著差异,并且均表现为:枝叶〉花〉果实。不同寄主的鞘花寄生,其枝叶、花和果实总黄酮含量分别为:5.29%~14.09%,3.92%~6.11%和2.68%-4.98%。(3)鞘花寄生总黄酮含量较高,极具开发利用价值。
為瞭開髮利用桂西北巖溶地區豐富的藥用植物資源,利用超聲波協同提取和正交試驗,優化鞘花寄生總黃酮提取的工藝條件。利用分光光度法,對陰香、香樟、桂花等7種寄主的鞘花寄生枝葉、花及果實總黃酮含量進行測定。結果錶明:(1)鞘花寄生果實總黃酮提取的優化工藝條件為:乙醇濃度30%,超聲波提取時間60min,超聲波提取功率180W,料液比1:30。在此條件下得到的鞘花寄生果實總黃酮提取率為3.96%。(2)不同寄主的鞘花寄生,其總黃酮含量有明顯差異;3n同寄主的鞘花寄生,其不同器官的總黃酮含量也有極顯著差異,併且均錶現為:枝葉〉花〉果實。不同寄主的鞘花寄生,其枝葉、花和果實總黃酮含量分彆為:5.29%~14.09%,3.92%~6.11%和2.68%-4.98%。(3)鞘花寄生總黃酮含量較高,極具開髮利用價值。
위료개발이용계서북암용지구봉부적약용식물자원,이용초성파협동제취화정교시험,우화초화기생총황동제취적공예조건。이용분광광도법,대음향、향장、계화등7충기주적초화기생지협、화급과실총황동함량진행측정。결과표명:(1)초화기생과실총황동제취적우화공예조건위:을순농도30%,초성파제취시간60min,초성파제취공솔180W,료액비1:30。재차조건하득도적초화기생과실총황동제취솔위3.96%。(2)불동기주적초화기생,기총황동함량유명현차이;3n동기주적초화기생,기불동기관적총황동함량야유겁현저차이,병차균표현위:지협〉화〉과실。불동기주적초화기생,기지협、화화과실총황동함량분별위:5.29%~14.09%,3.92%~6.11%화2.68%-4.98%。(3)초화기생총황동함량교고,겁구개발이용개치。
In order to develop the rich resources of medicinal plant in the karst area of northwest of Guan- gxi, the ultrasonic collaborative extraction method and orthogonal design were used to optimize the flavonoids ex- traction conditions of Macrosolen cochinchinensis. Flavonoids contained in M. cochinchinensis branches and leaves, flowers and fruits which are collected from seven host plants, such as Cinrtamomum burmannii, C. camphora and Osmanthus fragrans are determined by spectrophotometry respectively. The results show that the optimum flavonoids extraction technological conditions of M. cochinchinensis fruits are as follows: ethanol concentration 30%, ultrason- ic extraction time 60 min, ultrasonic extraction power 180 W, and material to extraction solution 1:30. Under the conditions, the extraction rate of the fruits is 3.96%. Secondly, flavonoids content of M. cochinchinensis in differ- ent hosts has evident differences, the flavonoids content of different organs of M. cochinchinensis in the same hosts also has significant differences. The order of flavonoids content from the maximum value to minimum value is: branches and leaves 〉 flowers 〉 fruits. The respective flavonoids contents of different hosts of M. cochinchinensis are : branches and leaves 5.29 % - 14.09 %, flowers 3.92% - 6.11%, and fruits 2.68 % ~ 4.98 %. Thirdly, the flavonoids content of M. cochinchinensis is higher, so it has great development and utilization value.