CAJ | 학술논문

对沾化冬枣根际解无机磷细菌进行了分离、筛选与鉴定，并对获得的高效溶磷菌进行培养条件及溶磷特性研究。利用透明圈法对沾化冬枣根际土壤中具有溶磷能力的细菌进行初筛；利用改良蒙金娜液体培养基对初筛菌株进行培养后采用钼锑抗比色法测定发酵液的可溶性磷含量，对解磷菌进行复筛；通过形态指标、16S rDNA 序列分析对该菌株进行了初步鉴定；同时对该溶磷菌株进行了耐盐试验、液体培养条件下溶磷特性研究、发酵培养基的优化试验，以及促进土壤中有效磷含量增加等一系列研究。结果显示，本试验从沾化冬枣根际土壤中分离出1株耐盐高效解无机磷细菌 PSB6，初步鉴定该菌株为土壤杆菌属，能显著增加发酵液和土壤中有效磷的含量。为通过生物途径改善沾化冬枣磷素供应，促进其生长提供了优良的菌株资源。
대첨화동조근제해무궤린세균진행료분리、사선여감정，병대획득적고효용린균진행배양조건급용린특성연구。이용투명권법대첨화동조근제토양중구유용린능력적세균진행초사；이용개량몽금나액체배양기대초사균주진행배양후채용목제항비색법측정발효액적가용성린함량，대해린균진행복사；통과형태지표、16S rDNA 서렬분석대해균주진행료초보감정；동시대해용린균주진행료내염시험、액체배양조건하용린특성연구、발효배양기적우화시험，이급촉진토양중유효린함량증가등일계렬연구。결과현시，본시험종첨화동조근제토양중분리출1주내염고효해무궤린세균 PSB6，초보감정해균주위토양간균속，능현저증가발효액화토양중유효린적함량。위통과생물도경개선첨화동조린소공응，촉진기생장제공료우량적균주자원。
Phosphate-solubilizing bacterium (PSB) was isolated,screened and identified from the rhizospheric soil of Zhanhua winter jujube (Zizyphus jujuba Mill.),and phosphate-dissolving characteristics of PSB were deter -mined.By using the way of clear zone,phosphate-solubilizing bacteria from the rhizospheric soil were preliminarily isolated,and the high effective PSB was further screened by using molybdenum -antimony anti-spectrophotometric method.The PSB was identified by the 16S rDNA sequence analysis combined with morphological characteristics . We carried out the salt-tolerant test,phosphate-solubilizing characteristics of PSB and optimization of fermentation medium in liquid tricalcium phosphate medium .The phosphate-dissolving characteristics of PSB in the soil were car -ried out under the greenhouse .The results indicated that one strain of high effective PSB was screened and identified as Rhizobium sp.The strain had significant effects on improving the solubilizing phosphate content in the liquid me -dium and the soil.This study provided excellent strain resource for improving phosphorus supply of Zhanhua winter jujube and promoting its growth.