CAJ | 학술논문

利用黑麦基因组特异 SCAR 标记对小麦抗叶锈病近等基因系材料 TcLr45和其背景材料 Thatcher 进行 PCR扩增，在 TcLr45中获得了单一条带，而在 Thatcher 中则扩增出2条与其大小不同的条带。进一步利用49个小麦抗叶锈近等基因系进行扩增，经5％聚丙烯酰胺凝胶电泳分析，该片段为 TcLr45特有片段。经 TcLr45×Thatcher F2分离群体验证，该标记与 Lr45基因连锁距离较远。对该片段进行克隆测序，片段长度为650 bp，与该引物在黑麦中的扩增序列有差异（643 bp），进一步经 BLAST2对这2个序列分析，相似性为97％，表明该片段为由黑麦向小麦转移的外源片段。100个小麦品种检测中有16个品种扩增出与 TcLr45相同的条带。
이용흑맥기인조특이 SCAR 표기대소맥항협수병근등기인계재료 TcLr45화기배경재료 Thatcher 진행 PCR확증，재 TcLr45중획득료단일조대，이재 Thatcher 중칙확증출2조여기대소불동적조대。진일보이용49개소맥항협수근등기인계진행확증，경5％취병희선알응효전영분석，해편단위 TcLr45특유편단。경 TcLr45×Thatcher F2분리군체험증，해표기여 Lr45기인련쇄거리교원。대해편단진행극륭측서，편단장도위650 bp，여해인물재흑맥중적확증서렬유차이（643 bp），진일보경 BLAST2대저2개서렬분석，상사성위97％，표명해편단위유흑맥향소맥전이적외원편단。100개소맥품충검측중유16개품충확증출여 TcLr45상동적조대。
A SCAR marker of rye genome was used to amplified the TcLr 45 and the background material Thatcher.One unique band in TcLr45,and two different bands in Thatcher were implified .In addition,49 wheat leaf rust resistance near isogenic gene lines (NILs) were detected by the marker,the marker is specific for TcLr45 a-mong these genes.F2 segregate groups screening results showed a long distance genetic linkage between Lr45 and the marker.The band was cloned and sequenced ,the full length 650 bp,which was different from the sequence am -plified in rye (643 bp),compared the two sequences by BLAST2 soft,the homology is 97%.16 of 100 wheat varie-ties were amplified the same bands as TcLr45.