三峡环境与生态
三峽環境與生態
삼협배경여생태
CHONGQING ENVIRONMENTAL SCIENCE
2012年
6期
23-25,49
,共4页
高通量筛选%腈水解酶%烟酸%活性
高通量篩選%腈水解酶%煙痠%活性
고통량사선%정수해매%연산%활성
high throughput screening%nitrilase%Nicotinic acid%activity
腈水解酶可将3-氰基吡啶直接水解生成烟酸,应用高通量筛选法从30种腈水解酶中筛选出131粗酶作为催化剂,测得该酶的酶活17.6U/mL,比酶活35.2U/mg,转化率最高。在温度为30℃,pH为8.0,底物质量浓度为8～10g/L时,腈水解酶131能将3-氰基吡啶完全转化为烟酸,产物纯品收率为85%左右。并用质谱和核磁验证产物结构的正确性。为建立烟酸制备的绿色工艺提供了新的思路。
腈水解酶可將3-氰基吡啶直接水解生成煙痠,應用高通量篩選法從30種腈水解酶中篩選齣131粗酶作為催化劑,測得該酶的酶活17.6U/mL,比酶活35.2U/mg,轉化率最高。在溫度為30℃,pH為8.0,底物質量濃度為8～10g/L時,腈水解酶131能將3-氰基吡啶完全轉化為煙痠,產物純品收率為85%左右。併用質譜和覈磁驗證產物結構的正確性。為建立煙痠製備的綠色工藝提供瞭新的思路。
정수해매가장3-청기필정직접수해생성연산,응용고통량사선법종30충정수해매중사선출131조매작위최화제,측득해매적매활17.6U/mL,비매활35.2U/mg,전화솔최고。재온도위30℃,pH위8.0,저물질량농도위8～10g/L시,정수해매131능장3-청기필정완전전화위연산,산물순품수솔위85%좌우。병용질보화핵자험증산물결구적정학성。위건립연산제비적록색공예제공료신적사로。
Nicotinic acid can be produced through direct hydrolysis of 3-cyanopyridine by Nitrilase.By high throughput screening,Nitrilase 131 was selected from 30 nitrilases as an applicable catalyst.It was measured that the conversion rate was the highest when its enzyme activity was 17.6U/mL and its specific activity was 35.2U/mg.At a temperature of 30℃ and a pH value of 8.0,when the initial substrate concentration was 8 ~ 10 g·L-1,3-cyanopyridine could be transformed into nicotinic acid completely with a pure product yield about 85%.The product structure was validated by MS and NMR.This study provides a new idea for the green technology of nicotinic acid's preparation.
