CAJ | 학술논문

为了研究野生三色拟迷孔茵多糖体外抗氧化以及抗肿瘤活性，采用热水浸提、乙醇沉淀、反复冻融以及透析等方法制备多糖样品，用DPPH法和羟自由基法检测样品对DPPH自由基和羟自由基的清除活性，用MTT法检测样品对不同细胞系的生长抑制情况。结果表明，在5mg·mL-1时，对DPPH自由基的清除率达到84．7％，而对羟自由基的清除率为67．4％；样品多糖作用48h后，对A549、LoVo和L1210三种细胞系的半数抑制浓度分别为0．78，1．05，0．44mg·mL-1。野生三色拟迷孔茵多糖具有一定的清除自由基的能力和较强的细胞生长抑制作用。
위료연구야생삼색의미공인다당체외항양화이급항종류활성，채용열수침제、을순침정、반복동융이급투석등방법제비다당양품，용DPPH법화간자유기법검측양품대DPPH자유기화간자유기적청제활성，용MTT법검측양품대불동세포계적생장억제정황。결과표명，재5mg·mL-1시，대DPPH자유기적청제솔체도84．7％，이대간자유기적청제솔위67．4％；양품다당작용48h후，대A549、LoVo화L1210삼충세포계적반수억제농도분별위0．78，1．05，0．44mg·mL-1。야생삼색의미공인다당구유일정적청제자유기적능력화교강적세포생장억제작용。
In order to study the wild Daedaleopsis tricolor polysaccharide (DTPs) in vitro antioxidant and antitumor activity, hot water extraction, ethanol precipitation, repeated freezing and thawing and dialysis and other methods are applied to prepare polysaccharide samples, and the antioxidant of DTPs is tested by applying DPPH and hydroxyl radical scavenging activity. MTT assay is applied to detect samples on different cell lines inhibiting growth effect. The results show that DTPs to DPPH radical scavenging rate reached 84.7%, and the hydroxyl radical scavenging rate of 67.4% in 5 mg·mL-1 DTPs treated cells after 48 h, and A549, LoVo, and L1210 three cell line half inhibitory concentrations were 0.78, 1.05, 0.44 mg·mL-1. Daedaleopsis tricolor polysaccharide has certain ability of scavenging free radicals and strong cell growth inhibitory effect.