热带农业工程
熱帶農業工程
열대농업공정
TROPICAL AGRICULTURAL ENGINEERING
2012年
6期
8-11
,共4页
蝴蝶兰%组织培养%外植体%原球茎
蝴蝶蘭%組織培養%外植體%原毬莖
호접란%조직배양%외식체%원구경
Phalaenopsis aphrodita Reich.b.f.%tissue culture%explants%PLB
以蝴堞兰带节花梗为外植体。在改良MS(其中大量元素量减半,其余全量,下同)附加10mg/LBA、1mg/LNAA、20%CM的培养基上,经4-6周的培养,每个外植体可长出2-3株小苗,小苗叶片长至2cm左右后,又以无菌小苗叶、叶柄及茎段为外植体,在1/2MS+10mg/LBA+10mg/LAD+1mg/LGA+30mg/L苹果酸+1g/L蛋白胨+10%香蕉汁的培养基诱导原球茎:香蕉汁可以减少原球茎的褐死现象并促进原球茎的增殖。
以蝴堞蘭帶節花梗為外植體。在改良MS(其中大量元素量減半,其餘全量,下同)附加10mg/LBA、1mg/LNAA、20%CM的培養基上,經4-6週的培養,每箇外植體可長齣2-3株小苗,小苗葉片長至2cm左右後,又以無菌小苗葉、葉柄及莖段為外植體,在1/2MS+10mg/LBA+10mg/LAD+1mg/LGA+30mg/L蘋果痠+1g/L蛋白胨+10%香蕉汁的培養基誘導原毬莖:香蕉汁可以減少原毬莖的褐死現象併促進原毬莖的增殖。
이호첩란대절화경위외식체。재개량MS(기중대량원소량감반,기여전량,하동)부가10mg/LBA、1mg/LNAA、20%CM적배양기상,경4-6주적배양,매개외식체가장출2-3주소묘,소묘협편장지2cm좌우후,우이무균소묘협、협병급경단위외식체,재1/2MS+10mg/LBA+10mg/LAD+1mg/LGA+30mg/L평과산+1g/L단백동+10%향초즙적배양기유도원구경:향초즙가이감소원구경적갈사현상병촉진원구경적증식。
Took the band festival of Phalaenopsis aphrodita Reich.b.f. as explants, the modified MS (of which a large number of elements by the volume, the rest of the whole volume, similarly hereinafter) with 10 mg/LBA, 1 mg/L NAA, 20% CM culture medium, 4-6 week later, each explant can grew 2-3 strains seedling, seedling leaves grew to around 2 cm, with sterile seedling leaves, petioles and stems as explants, in 1/2 MS+10 mg/L BA+10 mg/L AD+I mg/L GA+30 mg/L +18 L malic acid peptone + 10% banana juice culture medium inducing PLB PLB; banana juice can reduced the brown dead phenomenon and promoted PLB proliferation.