热带农业工程
熱帶農業工程
열대농업공정
TROPICAL AGRICULTURAL ENGINEERING
2012年
6期
4-7
,共4页
雒淑珍%于红霞%赵继荣%魏玉杰%陈调军%张梅秀%王军强
雒淑珍%于紅霞%趙繼榮%魏玉傑%陳調軍%張梅秀%王軍彊
락숙진%우홍하%조계영%위옥걸%진조군%장매수%왕군강
野罂粟%吗啡%液相色谱法
野罌粟%嗎啡%液相色譜法
야앵속%마배%액상색보법
wild poppy%morphine%liquid chromatography
采用HPLC测定罂粟茎、叶、壳部位中吗啡的含量,其色谱条件为:色谱柱HypersillC18(5μm,4.6nm×250nm):流动相:磷酸二氢钾(0.05mol/L):庚烷磺酸钠(0.0025mol/L):乙腈=(5:5:2);检测波长220nm。结果显示:罂粟植株中吗啡含量依次为:壳〉叶〉茎〉;罂粟壳中吗啡的含量为2.567%-3.455%,叶中含量为1.742%-2.653%,茎杆中含量为0.128%-0.467%。
採用HPLC測定罌粟莖、葉、殼部位中嗎啡的含量,其色譜條件為:色譜柱HypersillC18(5μm,4.6nm×250nm):流動相:燐痠二氫鉀(0.05mol/L):庚烷磺痠鈉(0.0025mol/L):乙腈=(5:5:2);檢測波長220nm。結果顯示:罌粟植株中嗎啡含量依次為:殼〉葉〉莖〉;罌粟殼中嗎啡的含量為2.567%-3.455%,葉中含量為1.742%-2.653%,莖桿中含量為0.128%-0.467%。
채용HPLC측정앵속경、협、각부위중마배적함량,기색보조건위:색보주HypersillC18(5μm,4.6nm×250nm):류동상:린산이경갑(0.05mol/L):경완광산납(0.0025mol/L):을정=(5:5:2);검측파장220nm。결과현시:앵속식주중마배함량의차위:각〉협〉경〉;앵속각중마배적함량위2.567%-3.455%,협중함량위1.742%-2.653%,경간중함량위0.128%-0.467%。
Determined the morphine content of poppy stems, leaves, shell parts by using the HPLC method, the chromatographic conditions were hypersillC18 (5 um, 4.6 nm.250 nm); mobile phase : potassium dihydrogen phosphate ( 0.05 mol/L ) : sodium heptanesulfonate ( 0.0 025 mol/L ) : acetonitrile = (5 : 5 : 2); detecting wavelength 220 nm. The results showed that the poppy plant morphine content in turn were shell 〉 leaf 〉 stem 〉; the morphine content of poppy shell was 2.567%-3.455%, in leaves was 1.742%-2.653%, in stem was 0.128%-0.467%.