CAJ | 학술논문

以高温豆粕为原料，通过单因素试验、正交试验进行直观分析和方差分析，确定碱性蛋白酶酶解高温豆粕的优化条件。以大豆肽水解度为指标，考察温度、时间、pH、加酶量、底物浓度等因素对水解度的影响。优化的酶解条件为：温度55℃、酶解时间为4h、pH为9．0、加酶量为9600U／g（底物）、底物浓度为10%，在此条件下做验证试验得到的大豆肽水解度为34．93％。
이고온두박위원료，통과단인소시험、정교시험진행직관분석화방차분석，학정감성단백매매해고온두박적우화조건。이대두태수해도위지표，고찰온도、시간、pH、가매량、저물농도등인소대수해도적영향。우화적매해조건위：온도55℃、매해시간위4h、pH위9．0、가매량위9600U／g（저물）、저물농도위10%，재차조건하주험증시험득도적대두태수해도위34．93％。
Taking high temperature bean pulp as raw material, single factor test, orthogonal. to the visual analysis and variance analysis to determine the alkaline protease enzyme optimal tem- perature conditions for soybean meal. Degree of hydrolysis of soybean peptide as an indicator of temperature, time, PH, enzyme amount, substrate concentration on the impact of the degree of hydrolysis. Optimization of hydrolysis conditions." temperature: 55 ℃, time: 4 h, pH: 9.0, en- zyme dosage: 9 600 U. g^-1 (substrate), substrate concentration: 10%. In this condition, the high temperature hydrolysis to confirm the test meal obtained soybean peptide hydrolysis was 34.93 %.