CAJ | 학술논문

通过硫酸铵分级沉淀、Q．Sepharose阴离子交换层析和SP-Sepharose阳离子交换层析等方法，从蓝圆鲣骨骼肌中分离纯化得到葡萄糖-6-磷酸异构酶（GPI）．SDS-PAGE结果显示，GPI分子质量约为56ku；Western-blot分析显示，纯化的蛋白质与抗白姑鱼GPI多克隆抗体有很强的阳性反应，表明其为GPI；GPI的最适pH值和最适温度分别为8．0和40oC；GPI能有效抑制蓝圆碜肌原纤维蛋白在55℃下的自身降解，且随着GPI添加浓度的增加，肌原纤维蛋白的降解逐渐受到抑制，提示GPI可用于鱼糜制品生产以提高产品的凝胶强度．
통과류산안분급침정、Q．Sepharose음리자교환층석화SP-Sepharose양리자교환층석등방법，종람원견골격기중분리순화득도포도당-6-린산이구매（GPI）．SDS-PAGE결과현시，GPI분자질량약위56ku；Western-blot분석현시，순화적단백질여항백고어GPI다극륭항체유흔강적양성반응，표명기위GPI；GPI적최괄pH치화최괄온도분별위8．0화40oC；GPI능유효억제람원삼기원섬유단백재55℃하적자신강해，차수착GPI첨가농도적증가，기원섬유단백적강해축점수도억제，제시GPI가용우어미제품생산이제고산품적응효강도．
Glucose-6-phosphate isomerase (GPI) from the blue scad skeletal muscle was purified to homogeneity by ammonium sulfate fractionation, column chromatographies of Q-Sepharose and SP-Sepharose. Purified GPI revealed a single band on SDS-PAGE with molecular weight of 56 ku. Western-blot analysis revealed that the purified protein from blue scad reacted positively with the polyclonal antibody against GPI from white croaker, suggesting this protein was GPI. The optimum pH and temperature of blue scad GPI were 8.0 and 40 ℃, respectively. At 55 ℃, with the increasing addition of GPI, the degradation of myofibrillar proteins was effectively inhibited, suggesting GPI could be applied for surimi production in order to improve the elasticity of the product.