集美大学学报：自然科学版
集美大學學報：自然科學版
집미대학학보：자연과학판
Journal of Jimei University(Natural Science)
2012年
6期
408-413
,共6页
马冬艳%吴维鹏%朱小明%魏虎进
馬鼕豔%吳維鵬%硃小明%魏虎進
마동염%오유붕%주소명%위호진
生态综合养殖%4’6-二脒基-2-苯基引哚(DAPI)%细菌丰度%水质因子
生態綜閤養殖%4’6-二脒基-2-苯基引哚(DAPI)%細菌豐度%水質因子
생태종합양식%4’6-이미기-2-분기인타(DAPI)%세균봉도%수질인자
Integrated Multi-trophic Aquaculture (IMTA)%DAPI%bacterial abundance%water factors
2011年4—10月在福建云霄，对以贝类为主的生态综合养殖系统内的藻类池（A池）和贝类池（B池）逐月采样检测，并分析两池水体及B池沉积物中细菌丰度的时空变化规律．结果发现：水体中细菌丰度，B池为1．01×10^6-4．35×10^6cell／mL；A池为1．09×10^6-6．61×10^6cell／mL．A池和B池水体中细菌丰度没有明显的月变化规律，但昼夜变化明显．B池水体中细菌丰度与叶绿素a、总氮、总磷、温度和盐度的关系4—7月呈明显的正相关（r=0．984，n=4）；8月细菌丰度急剧下降，因采样期间有连续降雨，细菌丰度变化和降雨可能有-定的关系．A池水体中细菌丰度与叶绿素a呈显著负相关（r=-0．821，n=7），与温度呈显著负相关（r=-0．830，n=7），温度不是影响A池细菌增殖的主要因子．B池底泥样品中细菌丰度比水体中的低，主要可能是水交换和养殖贝类活动共同作用的结果．采样期间发现，在阴雨天气时池塘水面会有泡沫漂浮，细菌丰度高于其所在水体达2．25×10^7cell／mL，其作用机制有待于进一步研究．
2011年4—10月在福建雲霄，對以貝類為主的生態綜閤養殖繫統內的藻類池（A池）和貝類池（B池）逐月採樣檢測，併分析兩池水體及B池沉積物中細菌豐度的時空變化規律．結果髮現：水體中細菌豐度，B池為1．01×10^6-4．35×10^6cell／mL；A池為1．09×10^6-6．61×10^6cell／mL．A池和B池水體中細菌豐度沒有明顯的月變化規律，但晝夜變化明顯．B池水體中細菌豐度與葉綠素a、總氮、總燐、溫度和鹽度的關繫4—7月呈明顯的正相關（r=0．984，n=4）；8月細菌豐度急劇下降，因採樣期間有連續降雨，細菌豐度變化和降雨可能有-定的關繫．A池水體中細菌豐度與葉綠素a呈顯著負相關（r=-0．821，n=7），與溫度呈顯著負相關（r=-0．830，n=7），溫度不是影響A池細菌增殖的主要因子．B池底泥樣品中細菌豐度比水體中的低，主要可能是水交換和養殖貝類活動共同作用的結果．採樣期間髮現，在陰雨天氣時池塘水麵會有泡沫漂浮，細菌豐度高于其所在水體達2．25×10^7cell／mL，其作用機製有待于進一步研究．
2011년4—10월재복건운소，대이패류위주적생태종합양식계통내적조류지（A지）화패류지（B지）축월채양검측，병분석량지수체급B지침적물중세균봉도적시공변화규률．결과발현：수체중세균봉도，B지위1．01×10^6-4．35×10^6cell／mL；A지위1．09×10^6-6．61×10^6cell／mL．A지화B지수체중세균봉도몰유명현적월변화규률，단주야변화명현．B지수체중세균봉도여협록소a、총담、총린、온도화염도적관계4—7월정명현적정상관（r=0．984，n=4）；8월세균봉도급극하강，인채양기간유련속강우，세균봉도변화화강우가능유-정적관계．A지수체중세균봉도여협록소a정현저부상관（r=-0．821，n=7），여온도정현저부상관（r=-0．830，n=7），온도불시영향A지세균증식적주요인자．B지저니양품중세균봉도비수체중적저，주요가능시수교환화양식패류활동공동작용적결과．채양기간발현，재음우천기시지당수면회유포말표부，세균봉도고우기소재수체체2．25×10^7cell／mL，기작용궤제유대우진일보연구．
Based on monthly sampling and examining the water and sediments of mulfi-trophic aquaculture (IMTA) systems which cultured shellfish (B pond) and algae (A pond) from April to October 2011 in Yunxiao County, Zhangzhou, Fujian Province. The temporal and spatial variation of bacteria abundance of these TMTA systems was discussed. The results showed that the bacteria abundance in B pond water counted through DAPI staining-fluorescence microscopy ranged from 1.01 ×10^6 to 4. 35 ×10^6 cell/mL, as for the A pond, the abundance ranged form 1.09 ×10^6 to 6. 61 ×10^6 cell/mL. There was no significant monthly variation found for the bacteria abundances in the water of IMTA systems. However, there was significant diurnal variation. The bacterial abundance in the B pond water from April to October was not significantly positive correlated to chlorophyll a, total nitrogen,, total phosphorus, temperature and salinity. However, significant positive correlation was found between bacterial abundance and temperature from April to July (r = 0. 984, n = 4) . Bacterial abundance increased with the temperature from April to July, but it decresed sharply in August, probobly it was due to the continuous rainfall. The bacterial abundance in the A pond water from April to October was significantly negative correlated to chlorophyll a (r = -0. 821 ,n = 7) and temperature (r = - 0. 830,n = 7) . Temperature was not the major factor for the bacterial abundance in the A pond water. It was found that the bacterial abundance of the sediments was lower than that in the B pond water, it was probably due to the combined action of the water exchange and shellfish activity. At the sampling period, some bubbles was found on the water surface in the rainy days, the bacterial abundance in the bubbles, reached up to 2. 25 ×10^7 cell/mL, much higher than the bacteria abundance in the water. However, the mechanism need to be further studied.
