CAJ | 학술논문

建立同时测定秦艽中龙胆苦苷和牛膝中蜕皮甾酮含量的RP—HPLC分析方法。采用KromacilC18色谱柱（4．6mm×250mm，5μm）；流动相：乙腈（A）-0．1％醋酸水溶液（B）进行梯度洗脱；流速1．0mL·min^-1；检测波长：254nm；柱温：25℃。龙胆苦苷、蜕皮甾酮的进样浓度分别在0．80～20．00，0．40～10．00μg·mL^-1。范围内线性关系良好；平均回收率（n=6）分别为100．02％，99．95％。结论：该方法简便，准确，重现性好，具有较强的应用价值。
건립동시측정진구중룡담고감화우슬중세피치동함량적RP—HPLC분석방법。채용KromacilC18색보주（4．6mm×250mm，5μm）；류동상：을정（A）-0．1％작산수용액（B）진행제도세탈；류속1．0mL·min^-1；검측파장：254nm；주온：25℃。룡담고감、세피치동적진양농도분별재0．80～20．00，0．40～10．00μg·mL^-1。범위내선성관계량호；평균회수솔（n=6）분별위100．02％，99．95％。결론：해방법간편，준학，중현성호，구유교강적응용개치。
To develop an HPLC method for determining the content of gentiopicrin and ecdysterone in Gentiana macrophyUa Pall and Achyranthes bidentata B1.A Kromacil Cls column (4.6mm×250mm,5μm) was adopted,the mobile phase was acetonitrile-water (contained 0.1% acetic acid) for gradient elution with flow rate of 1.0 mL·min^-1.The detection was set at 254 nm,and the column temperature was maintained at 25 ℃.Good linearities were obtained from 0.80 to 20.00,0.40 to 10.00 μg·mL^-1 for gentiopicrin and ecdysterone,respectively. The mean recoveries (n=6) of 2 components were 100.02%,99.95%,respectively. Conclusion: The method is simple and accurate with good reproducibility.