CAJ | 학술논문

以杂交得到的红皮红肉型火龙果自然成熟果实的种子为外植体,进行了快繁试验,筛选适宜的培养基、激素浓度配比,并对组培苗进行了移栽。结果显示,萌芽诱导培养1/2 MS＋NAA0.3 mg/L,实生苗增殖培养MS＋6-BA 5.0 mg/L＋NAA 0.3 mg/L,幼苗生长势强,质量好。子叶二次萌芽培养1/2 MS＋6-BA 1.5 mg/L,生根培养1/2 MS＋NAA 0.3 mg/L＋IBA 0.3 mg/L,生根率达95%。炼苗后7 d的幼苗移栽至处理好的苗床上,20 d后成活率为96%。
이잡교득도적홍피홍육형화룡과자연성숙과실적충자위외식체,진행료쾌번시험,사선괄의적배양기、격소농도배비,병대조배묘진행료이재。결과현시,맹아유도배양1/2 MS＋NAA0.3 mg/L,실생묘증식배양MS＋6-BA 5.0 mg/L＋NAA 0.3 mg/L,유묘생장세강,질량호。자협이차맹아배양1/2 MS＋6-BA 1.5 mg/L,생근배양1/2 MS＋NAA 0.3 mg/L＋IBA 0.3 mg/L,생근솔체95%。련묘후7 d적유묘이재지처리호적묘상상,20 d후성활솔위96%。
Tile stem segments with natural ripe fruit seeds of the red Pitaya hybrid offspring were used as experimental explants to study the different disinfection methods and the effects of hormone on adventitious bud occurrence and multiplication. The results indicated that: the medium of 1/2MS+NAA0.3 mg/L was best for bud infection. The optimal multiplication medimn in subculture was MS+6-BAS.0 mg/L+NAA0.3 rag/L, the multiplication rate was above 4.9, and the new bud had strong growth potential. The medium of 1/2MS +6-BA1.5 mg/L was best for the second bud infection of cotyledon. 1/2MS+NAA0.3mg/L+IBAO.3 mg/L was best medium for rooting, with a rooting of 95 %. After acclimatization for 7 days, the survival rate was up to 96 % on 20 days after transplanted into sterilized medium.