CAJ | 학술논문

建立了液相色谱-串联质谱法测定饲料中黄霉素A含量的检测方法,样品中的黄霉素A经氨水甲醇提取,HLB柱净化后,以Agilent Ecillps C18色谱柱为分析柱,乙腈-10 mmol/L的乙酸铵为流动相进行梯度洗脱,经液相色谱-串联四级杆质谱多反应监测（MRM）模式下检测。该方法黄霉素A的线性范围为100～5000μg/L,相关系数（r）为0.999725。依据S/N≥10确定方法的定量限为100μg/kg。添加浓度在100～1000μg/kg时,回收率在75%～95%之间,相对标准偏差（RSD）在4.7%～6.4%之间（n=5）。
건립료액상색보-천련질보법측정사료중황매소A함량적검측방법,양품중적황매소A경안수갑순제취,HLB주정화후,이Agilent Ecillps C18색보주위분석주,을정-10 mmol/L적을산안위류동상진행제도세탈,경액상색보-천련사급간질보다반응감측（MRM）모식하검측。해방법황매소A적선성범위위100～5000μg/L,상관계수（r）위0.999725。의거S/N≥10학정방법적정량한위100μg/kg。첨가농도재100～1000μg/kg시,회수솔재75%～95%지간,상대표준편차（RSD）재4.7%～6.4%지간（n=5）。
In orderto establish a liquid chromatography tandem mass spectrometry determination the content of flavomycin A in feed,the flavomycin A in sample extracted by ammonia methanol and purified by HLB column,Agilent Ecillps C18 chromatographic column as analysis column,acetonitrile-10 mmol/L ammonium acetate as gradient elution solution,then determined at multiple reaction monitoring(MRM) by liquid chromatography tandem mass spectrometry,The linear range of flavomycin A was 100~5000 μg/L,correlation coefficient(r) was 0.999725.According to S/N≥10 determination of the quantitation limit of flavomycin A in the method is 100 μg/kg when the additive concentration at the range of 100~1000 μg/kg,the mean recoveries ranged between 75% and 95%,and the relative standard deviations(RSD) between 4.7% and 6.4%(n=5).